Comparison of the Effect of Heterologous and Homologous Seminal Plasma on Motility and Chromatin Integrity of Stallion Spermatozoa Selected by Single Layer Centrifugation

• The effect on sperm motility and chromatin integrity of adding homologous or heterologous equine seminal plasma (SP) to freshstallion spermatozoa selected by single layer centrifugation (SLC) was studied.No statistical difference in mean progressivemotilitywas seen after adding SP at time 0 h, although there were differences for individual stallions.The proportion of spermatozoa withhigh velocity was increased compared to untreated SLC-selected spermatozoa (�� < 0.05), with significant differences betweenindividuals (�� < 0.01). When the SLC samples were stored for 24 h before adding SP, a significant increase in mean progressivemotility was seen for SLC + homologous SP (�� < 0.01) and for SLC + heterologous SP (�� < 0.056). Whether homologous SPor heterologous SP had a greater effect on progressive motility depended on the individual. Adding either type of SP caused asignificant increase in chromatin damage compared to SLC after storage for 24 h (homologous SP, �� < 0.05; heterologous SP,�� < 0.01). These preliminary data showed that storage of SLC-spermatozoa mixed with SP should be avoided because of the risk ofincreased chromatin damage. If SP is to be added to take advantage of a transient increase in progressive motility for a particularindividual stallion, different combinations of SP and spermatozoa should be tested first to optimize the effect.

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LANTBRUKSVETENSKAPER  -- Veterinärmedicin -- Annan veterinärmedicin (hsv//swe)

AGRICULTURAL SCIENCES  -- Veterinary Science -- Other Veterinary Science (hsv//eng)

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