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Heterotrimeric G-pr...
Heterotrimeric G-proteins in Picea abies and their regulation in response to Heterobasidion annosum s.l. infection
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- de Vries, Sophie (författare)
- Heinrich Heine University Düsseldorf
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- Nemesio Gorriz, Miguel (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Department of Forest Mycology and Plant Pathology
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- Karlsson, Magnus (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig mykologi och patologi,Department of Forest Mycology and Pathology
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- Elfstrand, Malin (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig mykologi och patologi,Department of Forest Mycology and Pathology
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(creator_code:org_t)
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- 2015-12-12
- 2015
- Engelska.
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Ingår i: BMC Plant Biology. - : Springer Science and Business Media LLC. - 1471-2229. ; 15, s. 1-15
- Relaterad länk:
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https://bmcplantbiol...
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https://res.slu.se/i...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Background: Heterotrimeric G-proteins are important signalling switches, present in all eukaryotic kingdoms. In plants they regulate several developmental functions and play an important role in plant-microbe interactions. The current knowledge on plant G-proteins is mostly based on model angiosperms and little is known about the G-protein repertoire and function in other lineages. In this study we investigate the heterotrimeric G-protein subunit repertoire in Pinaceae, including phylogenetic relationships, radiation and sequence diversity levels in relation to other plant linages. We also investigate functional diversification of the G-protein complex in Picea abies by analysing transcriptional regulation of the G-protein subunits in different tissues and in response to pathogen infection.Results: A full repertoire of G-protein subunits in several conifer species were identified in silico. The full-length P. abies coding regions of one G alpha-, one G beta -and four G gamma-subunits were cloned and sequenced. The phylogenetic analysis of the G gamma-subunits showed that PaGG1 clustered with A-type-like subunits, PaGG3 and PaGG4 clustered with C-type-like subunits, while PaGG2 and its orthologs represented a novel conifer-specific putative G gamma-subunit type. Gene expression analyses by quantitative PCR of P. abies G-protein subunits showed specific up-regulation of the G alpha-subunit gene PaGPA1 and the G gamma-subunit gene PaGG1 in response to Heterobasidion annosum sensu lato infection.Conclusions: Conifers possess a full repertoire of G-protein subunits. The differential regulation of PaGPA1 and PaGG1 indicates that the heterotrimeric G-protein complex represents a critical linchpin in Heterobasidion annosum s.l. perception and downstream signaling in P. abies.
Ämnesord
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
- LANTBRUKSVETENSKAPER -- Lantbruksvetenskap, skogsbruk och fiske -- Skogsvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Agriculture, Forestry and Fisheries -- Forest Science (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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