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Inter-laboratory ev...
Inter-laboratory evaluation of SEC-post-column calcofluor for determination of the weight-average molar mass of cereal β-glucan
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- Andersson, Roger (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för livsmedelsvetenskap,Department of Food Science
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(creator_code:org_t)
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- Elsevier BV, 2015
- 2015
- Engelska.
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Ingår i: Carbohydrate Polymers. - : Elsevier BV. - 0144-8617. ; 124, s. 254-264
- Relaterad länk:
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https://res.slu.se/i...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Even though size exclusion chromatography (SEC) with post column addition of calcofluor (SEC-calcofluor) has been used for the determination of cereal beta-glucan molar mass in foods for many years, there is a lack of systematic evaluation of the method. To address this issue a set of suitable beta-glucan standards were generated by preparative SEC and their molar mass characteristics were determined by analytical multi-detection SEC (refractive index (RI), light scattering). Each standard was then analysed by SEC-calcofluor at three different labs. As a direct comparison, the analyses were repeated with a RI detector. For SEC-calcofluor accurate measurements of weight average molar mass (M-w) can be made for beta-glucan populations within 10-500 x 10(3) g/mol. Above this molar mass threshold there is an increasing tendency for underestimation of M-w. Precipitation of some beta-glucan-calcofluor complexes may have delayed their transport into the detector. (C) 2015 Elsevier Ltd. All rights reserved.
Ämnesord
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
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