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Isolation of Apoplast
Isolation of Apoplast
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- Andreasson, Erik (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Växtskyddsbiologi,Department of Plant Protection Biology
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- Abreha, Kibrom Berhe (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Växtskyddsbiologi,Department of Plant Protection Biology
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- Resjö, Svante (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Växtskyddsbiologi,Department of Plant Protection Biology
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(creator_code:org_t)
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- ISBN 9781493965311
- 2016-10-12
- 2017
- Engelska.
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Ingår i: Methods in Molecular Biology. - New York, NY : Springer New York. - 1064-3745 .- 1940-6029. ; 1511, s. 233-240
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Innehållsförteckning
Abstract
Ämnesord
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- The apoplast can be described as the soluble fraction of the extracellular space of plant tissue, and it plays an important role in signaling, nutrient transport, and plant-pathogen interactions. In this protocol, we describe a method where leaves are infiltrated with phosphate buffer under vacuum. The apoplast can then be extracted by centrifugation and simultaneously collected in a protease inhibitor solution. Using this protocol, typically 3 mu g of apoplastic proteins can be obtained in a volume of 300 mu L from five potato leaflets, with minimal contamination by non-apoplastic proteins.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
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