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> Tegenfeldt Jonas O. >
A lipid-based passi...
A lipid-based passivation scheme for nanofluidics
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- Fritzsche, Joachim, 1977 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU),University of Gothenburg
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- Persson, Fredrik (author)
- Uppsala University,University of Gothenburg
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- Mir, K. U. (author)
- Wellcome Trust Centre for Human Genetics
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- Modesti, M. (author)
- Aix-Marseille Université,Aix Marseille University,Aix-Marseille University
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- Westerlund, Fredrik, 1978 (author)
- Chalmers University of Technology
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- Tegenfeldt, Jonas O. (author)
- Lund University,Lunds universitet,Gothenburg University,Göteborgs universitet,Institutionen för fysik (GU),Department of Physics (GU),University of Gothenburg,NanoLund: Centre for Nanoscience,Annan verksamhet, LTH,Lunds Tekniska Högskola,Fasta tillståndets fysik,Fysiska institutionen,Institutioner vid LTH,Other operations, LTH,Faculty of Engineering, LTH,Solid State Physics,Department of Physics,Departments at LTH,Faculty of Engineering, LTH
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(creator_code:org_t)
- ISBN 9780979806452
- 2012
- 2012
- English.
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In: 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012; Okinawa; Japan; 28 October 2012 through 1 November 2012. - 9780979806452 ; , s. 1876-1878
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Abstract
Subject headings
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- Stretching DNA in nanochannels allows for direct, visual studies of genomic DNA at the single molecule level. In order to facilitate the study of the interaction of linear DNA with proteins in nanochannels, we have implemented a highly effective passivation scheme based on lipid bilayers. We show long-term passivation of nanochannel surfaces to several relevant reagents and demonstrate that the performance of the lipid bilayer is significantly better compared to standard bovine serum albumin-based passivation. Moreover, we demonstrate how the passivated devices allow us to monitor single DNA cleavage events during enzymatic degradation.
Subject headings
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
- NATURVETENSKAP -- Fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
Keyword
- Lipid bilayer
- Nanofluidics
- Passivation
- Protein-DNA interactions
- Single molecules
- DNA
- Lipid bilayers
- Molecules
- Proteins
- Bovine serum
- DNA cleavage
- Enzymatic Degradation
- Genomic DNA
- Nano channels
- Single molecule
- Single molecule level
- Enzymatic Degradation
Publication and Content Type
- ref (subject category)
- kon (subject category)
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