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Users in Action. Stories of Users and Telematics in Everyday Life
- 1999
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Editorial collection (other academic/artistic)abstract
- Detta är en antologi som består av exempel på empiriska studier där äldre människors varit involverade som subjekt. Resultatet visar att äldres teknikanvändning i vardagen problematiseras på tre nivåer. den första nivån fokuserar konstruktionen och utformningen av artefakten; den andra nivån användningen av tekniken i den vardagliga kontexten; den tredje nivån relationen mellan teknikutvecklingen och användaren som samhällsvarelse.
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- Abel, Frida, 1974, et al.
(author)
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Gain of chromosome arm 17q is associated with unfavourable prognosis in neuroblastoma, but does not involve mutations in the somatostatin receptor 2(SSTR2) gene at 17q24.
- 1999
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In: British journal of cancer. - : Springer Science and Business Media LLC. - 0007-0920 .- 1532-1827. ; 81:8, s. 1402-9
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Journal article (peer-reviewed)abstract
- Deletion of chromosome arm 1p and amplification of the MYCN oncogene are well-recognized genetic alterations in neuroblastoma cells. Recently, another alteration has been reported; gain of the distal part of chromosome arm 17q. In this study 48 neuroblastoma tumours were successfully analysed for 17q status in relation to known genetic alterations. Chromosome 17 status was detected by fluorescence in situ hybridization (FISH). Thirty-one of the 48 neuroblastomas (65%) showed 17q gain, and this was significantly associated with poor prognosis. As previously reported, 17q gain was significantly associated with metastatic stage 4 neuroblastoma and more frequently detected than both deletion of chromosome arm 1p and MYCN amplification in tumours of all stages. 17q gain also showed a strong correlation to survival probability (P = 0.0009). However, the most significant correlation between 17q gain and survival probability was observed in children with low-stage tumours (stage 1, 2, 3 and 4S), with a survival probability of 100% at 5 years from diagnosis for children with tumours showing no 17q gain compared to 52.5% for those showing 17q gain (P = 0.0021). This suggests that 17q gain as a prognostic factor plays a more crucial role in low-stage tumours. Expression of the somatostatin receptor 2 (SSTR2), localized in chromosome region 17q24, has in previous studies been shown to be positively related to survival in neuroblastoma. A point mutation in the SSTR2 gene has earlier been reported in a human small-cell lung cancer. In this study, mutation screening of the SSTR2 gene in 43 neuroblastoma tumours was carried out with polymerase chain reaction-based single-stranded conformation polymorphism/heteroduplex (SSCP/HD) and DNA sequencing, and none of the tumours showed any aberrations in the SSTR2 gene. These data suggest that mutations in the SSTR2 gene are uncommon in neuroblastoma tumours and do not correlate with either the 17q gain often seen or the reason some tumours do not express SSTR2 receptors. Overall, this study indicates that gain of chromosome arm 17q is the most frequently occurring genetic alteration, and that it is associated with established prognostic factors.
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- Tamás, Markus J., 1970, et al.
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Fps1p controls the accumulation and release of the compatible solute glycerol in yeast osmoregulation.
- 1999
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In: Molecular microbiology. - 0950-382X .- 1365-2958. ; 4, s. 1087-104
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Journal article (peer-reviewed)abstract
- The accumulation of compatible solutes, such as glycerol, in the yeast Saccharomyces cerevisiae, is a ubiquitous mechanism in cellular osmoregulation. Here, we demonstrate that yeast cells control glycerol accumulation in part via a regulated, Fps1p-mediated export of glycerol. Fps1p is a member of the MIP family of channel proteins most closely related to the bacterial glycerol facilitators. The protein is localized in the plasma membrane. The physiological role of Fps1p appears to be glycerol export rather than uptake. Fps1 delta mutants are sensitive to hypo-osmotic shock, demonstrating that osmolyte export is required for recovery from a sudden drop in external osmolarity. In wild-type cells, the glycerol transport rate is decreased by hyperosmotic shock and increased by hypo-osmotic shock on a subminute time scale. This regulation seems to be independent of the known yeast osmosensing HOG and PKC signalling pathways. Mutants lacking the unique hydrophilic N-terminal domain of Fps1p, or certain parts thereof, fail to reduce the glycerol transport rate after a hyperosmotic shock. Yeast cells carrying these constructs constitutively release glycerol and show a dominant hyperosmosensitivity, but compensate for glycerol loss after prolonged incubation by glycerol overproduction. Fps1p may be an example of a more widespread class of regulators of osmoadaptation, which control the cellular content and release of compatible solutes.
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- Sohler, D., et al.
(author)
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Yrast states of the proton drip line nucleus 106 Sb
- 1999
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In: Physical Review C - Nuclear Physics. - 2469-9985 .- 2469-9993 .- 0556-2813 .- 1089-490X. ; 59:3, s. 1324-1327
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Journal article (peer-reviewed)abstract
- Yrast states of [Formula Presented] have been investigated in the [Formula Presented] reaction using in-beam [Formula Presented]-spectroscopic methods and in the [Formula Presented] reaction performing delayed [Formula Presented] and conversion electron studies. A new isomeric state was found at 103 keV with [Formula Presented] The number of states and transitions in the proposed level scheme have been doubled. The experimental results are discussed within the framework of the shell model. © 1999 The American Physical Society.
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- Pradhan, P., et al.
(author)
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Studies on the Adduct Heterogeneity of Benzo(a)pyrene 7,8-Dihydrodiol 9,10-Epoxide Stereoisomers Covalently Bound to Deoxyribooligonucleotides by Induced Circular Dichroism and Light Absorption Spectroscopy
- 1999
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In: Chemical Research in Toxicology. - : American Chemical Society (ACS). - 1520-5010 .- 0893-228X. ; 12:5, s. 403-411
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Journal article (peer-reviewed)abstract
- The binding conformations of single anti- and syn-BPDE-N-2-dG adducts in oligonucleotides of varying base composition have been studied by induced circular dichroism (ICD) and light absorption spectroscopy. The sign of the ICD in single-stranded oligonucleotide adducts correlates with the absolute configuration of the cyclohexyl moiety of the BPDE. Adducts in oligonucleotide duplexes with UV lambda(max) 350 nm exhibiting either positive or negative contributions to the ICD should have intercalated binding as the predominant conformation. The magnitude of the ICD is dependent on the sequence context of the adducted strand and the particular BPDE-adduct isomer under study. In some cases, the results suggest structural heterogeneity. For instance, the (+)- and the (-)-trans-anti-BPDE-N-2-dG adducts in duplexes where a dT flanks the lesion site exhibit weak positive ICD or negative ICD. These results reflect a bimodal conformational adduct distribution with contributions from both externally and internally located adducts. A key observation for the (+)-cis-syn-BPDE-N-2-dG complexes in 5'-d(TGC) and 5'-d(CGC) sequence contexts is that the near-UV absorption spectra showed distinct bands corresponding to minor groove binding (lambda(max) congruent to 346 nm) as well as intercalative binding (lambda(max) congruent to 354 nm). Evidence for an equilibrium between the different modes of localization is provided by the results from the temperature dependence of the near-UV absorption and ICD characteristics of(+)-cis-synBPDE-N-2-dG complexes in 5'-d(TGC) and 5'-d(CGC) sequence contexts, respectively.
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- Christiansson, Anna-Karin, 1947-, et al.
(author)
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Mixed continuous/discrete-time output feedback H∞ control : A unified approach
- 1999
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In: European Control Conference, ECC 1999 - Conference Proceedings. - 9783952417355 ; , s. 4077-4082
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Conference paper (peer-reviewed)abstract
- A Riccati equation (RE) based solution to the H∞ optimal control problem for mixed continuous/discrete-time systems is presented. The results unify a number of recently penetrated H∞ control problems. In the infinite-horizon case a periodic behaviour is assumed, and it is shown how the related continuous RE with jumps can be replaced by an equivalent discrete periodic RE. A related algebraic RE, which involves the system behaviour during one period, then can be formulated and solved by standard methods. Typical applications are control of continuous-time or discrete-time periodic systems, as well as multirate and sampled-data control, including mixed continuous and sampled-data measurements. © 1999 EUCA.
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