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- Kroca, Michal, et al.
(författare)
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Vγ9Vδ2 T cells in human legionellosis
- 2001
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Ingår i: Clinical and diagnostic laboratory immunology. - 1071-412X. ; 8, s. 949-954
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Tidskriftsartikel (refereegranskat)
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| 7. |
- Lai, Xin-He, et al.
(författare)
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Delineation of the molecular mechanisms of Francisella tularensis-induced apoptosis in murine macrophages
- 2003
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Ingår i: Infection and Immunity. - 0019-9567. ; 71:8, s. 4642-4646
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Tidskriftsartikel (refereegranskat)abstract
- Francisella tularensis is a facultative intracellular bacterium capable of inducing apoptosis in murine macrophages. Here we analyzed the pathway leading to apoptosis in the murine macrophage-like cell line J774A.1 after infection with F. tularensis strain LVS (named LVS for live vaccine strain). We obtained evidence that the infection affected the mitochondria of the macrophages, since it induced release of the mitochondrial molecule cytochrome c into the cytosol and changed the potential over the mitochondrial membrane. Moreover, activation of caspase 9 and the executioner caspase 3 was also observed in the LVS-infected J774A.1 macrophages. The activated caspase 3 degraded poly(ADP-ribose) polymerase (PARP). All of these events were observed within 9 to 12 h after the initiation of infection, and maximum degradation of a synthetic caspase 3 substrate occurred at 18 h. The internucleosomal fragmentation and PARP degradation resulting from activation of this apoptotic pathway was prevented by the caspase 3 inhibitor Z-DEVD-fmk. No involvement of caspase 1, caspase 8, Bcl-2, or Bid was observed. Thus, the F. tularensis infection induces macrophage apoptosis through a pathway partly resembling the intrinsic apoptotic pathway.
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- Lai, Xin-He, et al.
(författare)
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Expression of iglC is mecessary for intracellular growth and induction of apoptosis in murine macrophages by Francisella tularensis
- 2004
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Ingår i: Microbial Pathogenesis. - London : Academic P.. - 0882-4010. ; 37:5, s. 225-230
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Tidskriftsartikel (refereegranskat)abstract
- Francisella tularensis is a facultative intracellular bacterium capable of inducing apoptosis in murine macrophages. In a previous study, an iglC null mutant of F. tularensis live vaccine strain LVS was generated by allelic replacement and in the current study this iglC mutant was successfully complemented in trans. We characterized the capacity of this iglC mutant and the complemented strain to induce macrophage apoptosis. The iglC mutant did not induce apoptosis in the infected cells. In contrast, the complemented iglC strain was able to multiply in the murine macrophage-like cell line J774A.1 and induced apoptosis similar to that of the wild-type strain. It is the first successful example of complementation in trans of a F. tularensis mutant strain and more importantly this work provides direct evidence that the intracellular growth ability is essential for F. tularensis to induce macrophage apoptosis.
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- Lai, Xin-He, et al.
(författare)
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Fancisella tularensis induces cytopathogenicity and apaptosis in murine macrophages via a mechanism that requires intracellular bacterial multiplication
- 2001
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Ingår i: Infection and Immunity. - 0019-9567. ; 69:7, s. 4691-4694
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Tidskriftsartikel (refereegranskat)abstract
- The murine macrophage-like cell line J774.A1 ingests and allows intracellular growth of Francisella tularensis. We demonstrate that, after 24 h of infection, a pronounced cytopathogenicity resulted and the J774 cells were undergoing apoptosis. Despite this host cell apoptosis, no decrease in bacterial numbers was observed. When internalization of bacteria was prevented or intracellularly located F. tularensis bacteria were eradicated within 12 h, the progression of host cell cytopathogenicity and apoptosis was prevented.
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- Lai, Xin-He, et al.
(författare)
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Francisella strains express hemolysins of distinct characteristics
- 2003
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Ingår i: FEMS Microbiology Letters. - 0378-1097. ; 224:1, s. 91-95
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Tidskriftsartikel (refereegranskat)abstract
- Historically, Francisella strains have been described as nonhemolytic. In this study, we show by use of solid and liquid hemolysis assays that some Francisella strains have hemolytic properties. The Francisella novicida type strain U112 is hemolytic to horse erythrocytes and Francisella philomiragia type strain FSC144 is hemolytic towards both human and horse erythrocytes. The F. novicida strain U112 released a protein (novilysin A) into the culture supernatant which cross-reacted with antiserum against Escherichia coli HlyA whereas there was no similar protein detectable with this cross-reactive property from the supernatant of the F. philomiragia strain.
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