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Sökning: swepub > Umeå universitet > (2000-2004) > Södertörns högskola

  • Resultat 1-10 av 37
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1.
  • Schubert, Maria, et al. (författare)
  • Proteome Map of the Chloroplast Lumen of Arabidopsis thaliana
  • 2002
  • Ingår i: Journal of Biological Chemistry. - : American Society for Biochemistry and Molecular Biology. - 0021-9258 .- 1083-351X. ; 277:10, s. 8354-8365
  • Tidskriftsartikel (refereegranskat)abstract
    • The thylakoid membrane of the chloroplast is the center of oxygenic photosynthesis. To better understand the function of the luminal compartment within the thylakoid network, we have carried out a systematic characterization of the luminal thylakoid proteins from the model organism Arabidopsis thaliana. Our data show that the thylakoid lumen has its own specific proteome, of which 36 proteins were identified. Besides a large group of peptidyl-prolyl cis-trans isomerases and proteases, a family of novel PsbP domain proteins was found. An analysis of the luminal signal peptides showed that 19 of 36 luminal precursors were marked by a twin-arginine motif for import via the Tat pathway. To compare the model organism Arabidopsis with another typical higher plant, we investigated the proteome from the thylakoid lumen of spinach and found that the luminal proteins from both plants corresponded well. As a complement to our experimental investigation, we made a theoretical prediction of the luminal proteins from the whole Arabidopsis genome and estimated that the thylakoid lumen of the chloroplast contains ~80 proteins.
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2.
  • Gallio, Marco, et al. (författare)
  • Rhomboid 3 orchestrates Slit-independent repulsion of tracheal branches at the CNS midline.
  • 2004
  • Ingår i: Development. - : The Company of Biologists. - 0950-1991 .- 1477-9129. ; 131:15, s. 3605-3614
  • Tidskriftsartikel (refereegranskat)abstract
    • EGF-receptor ligands act as chemoattractants for migrating epithelial cells during organogenesis and wound healing. We present evidence that Rhomboid 3/EGF signalling, which originates from the midline of the Drosophila ventral nerve cord, repels tracheal ganglionic branches and prevents them from crossing it. rho3 acts independently from the main midline repellent Slit, and originates from a different sub-population of midline cells: the VUM neurons. Expression of dominant-negative Egfr or Ras induces midline crosses, whereas activation of the Egfr or Ras in the leading cell of the ganglionic branch can induce premature turns away from the midline. This suggests that the level of Egfr intracellular signalling, rather than the asymmetric activation of the receptor on the cell surface, is an important determinant in ganglionic branch repulsion. We propose that Egfr activation provides a necessary switch for the interpretation of a yet unknown repellent function of the midline.
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3.
  • Onischenko, Evgeny A, et al. (författare)
  • Annulate Lamellae Play Only a Minor Role in the Storage of Excess Nucleoporins in Drosophila Embryos
  • 2004
  • Ingår i: Traffic. - : Blackwell Munksgaard. - 1398-9219 .- 1600-0854. ; 5:3, s. 152-164
  • Tidskriftsartikel (refereegranskat)abstract
    • The nuclear pore complexes (NPCs), multiprotein assemblies embedded in the nuclear envelope, conduct nucleo-cytoplasmic traffic of macromolecules. Mimics of NPCs, called annulate lamellae pore complexes (ALPCs), are usually found in cytoplasmic membranous stacks in oocytes and early embryonic cells. They are believed to constitute storage compartments for excess premade nucleoporins. To evaluate the extent to which ALPCs store nucleoporins in early embryonic cells we took advantage of syncytial Drosophila embryos, containing both AL and rapidly proliferating nuclei in the common cytoplasm. Electron microscopic morphometric analysis showed that the number of ALPCs did not decrease to compensate for the growing number of NPCs during syncytial development. We performed Western blot analysis to quantify seven different nucleoporins and analyzed their intraembryonal distribution by confocal microscopy and subcellular fractionation. Syncytial embryos contained a large maternally contributed stockpile of nucleoporins. However, even during interphases, only a small fraction of the excess nucleoporins was assembled into ALPCs, whereas the major fraction was soluble and contained at least one phosphorylated nucleoporin. We conclude that in Drosophila embryos ALPCs play only a minor role in storing the excess maternally contributed nucleoporins. Factors that may prevent nucleoporins from assembly into ALPCs are discussed.
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4.
  • Thidholm, Ellinor, et al. (författare)
  • Novel approach reveals localisation and assembly pathway of the PsbS and PsbW proteins into the photosystem II dimer
  • 2002
  • Ingår i: FEBS LETTERS. - 0014-5793 .- 1873-3468. ; 513:2-3, s. 217-22
  • Tidskriftsartikel (refereegranskat)abstract
    • A blue-native gel electrophoresis system was combined with an in organello import assay to specifically analyse the location and assembly of two nuclear-encoded photosystem 11 (PSII) subunits. With this method we were able to show that initially the low molecular mass PsbW protein is not associated with the monomeric form of PSII. Instead a proportion of newly imported PsbW is directly assembled in dimeric PSH super-complexes with very fast kinetics; its negatively charged N-terminal domain is essential for this process. The chlorophyll-binding PsbS protein, which is involved in energy dissipation, is first detected in the monomeric PSII subcomplexes, and only at later time points in the dimeric form of PSII. It seems to be bound tighter to the PSII core complex than to light harvesting complex II. These data point to radically different assembly pathways for different PSII subunits. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
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5.
  • Bergman, Mats A., et al. (författare)
  • Large investments in the pulp and paper industry : A count-data regression analysis
  • 2002
  • Ingår i: Journal of Forest Economics. - : Now Publishers Inc.. - 1104-6899 .- 1618-1530. ; 8, s. 29-52
  • Tidskriftsartikel (refereegranskat)abstract
    • This paper analyses the effects of price and market size variables on the investment propensities in the pulp and paper industry. A panel of 15 European countries in the time period 1988-1997 is used in the regression analysis. We find the wages, the USD/ECU exchange rate, the price of paper and the installed production capacity to be the main determinants of large investments in this industry. Our measure of market size has no - or only very small - effects.
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6.
  • Aronsson, Thomas, 1963-, et al. (författare)
  • The impact of generic drug competition on brand name market shares - evidence from micro data
  • 2001
  • Ingår i: Review of Industrial Organization. - : Kluwer Academic Publishers. - 0889-938X .- 1573-7160. ; 19:4, s. 423-433
  • Tidskriftsartikel (refereegranskat)abstract
    • This paper analyses how market shares for brand name drugs are affected by generic competition. The analysis is based on micro data for twelve different original drugs, which are all subject to generic competition. For five of these drugs, we find that the price of the original relative to the average price of the generic substitutes significantly affects the market share of the original drug. In addition, the introduction of a so called "reference price" system appears to have had a significant impact on the market shares of five original drugs.
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9.
  • Bergman, Mats (författare)
  • Competition in services or infrastructure-based competition?
  • 2004
  • Ingår i: An anthology on the foundations for competition and development in electronic communications markets. - Stockholm : Post- och telestyrelsen. ; , s. 6-55
  • Bokkapitel (övrigt vetenskapligt/konstnärligt)
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10.
  • Bergman, Mats (författare)
  • Det amerikanska Microsoftfallet
  • 2001
  • Ingår i: Konkurrensnytt. - : Konkurrensverket. - 1404-7217. ; :11, s. 6-7
  • Tidskriftsartikel (populärvet., debatt m.m.)
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