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Sökning: swepub > Malmö universitet > Engelska > Shleev Sergey

  • Resultat 1-10 av 87
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  • Andoralov, Viktor, et al. (författare)
  • Biofuel cell based on microscale nanostructured electrodes with inductive coupling to rat brain neurons.
  • 2013
  • Ingår i: Scientific Reports. - Nature Publishing Group. - 2045-2322. ; 3:Nov 20
  • Tidskriftsartikel (refereegranskat)abstract
    • Miniature, self-contained biodevices powered by biofuel cells may enable a new generation of implantable, wireless, minimally invasive neural interfaces for neurophysiological in vivo studies and for clinical applications. Here we report on the fabrication of a direct electron transfer based glucose/oxygen enzymatic fuel cell (EFC) from genuinely three-dimensional (3D) nanostructured microscale gold electrodes, modified with suitable biocatalysts. We show that the process underlying the simple fabrication method of 3D nanostructured electrodes is based on an electrochemically driven transformation of physically deposited gold nanoparticles. We experimentally demonstrate that mediator-, cofactor-, and membrane-less EFCs do operate in cerebrospinal fluid and in the brain of a rat, producing amounts of electrical power sufficient to drive a self-contained biodevice, viz. 7 μW cm(-2) in vitro and 2 μW cm(-2) in vivo at an operating voltage of 0.4 V. Last but not least, we also demonstrate an inductive coupling between 3D nanobioelectrodes and living neurons.
  • Shleev, Sergey, et al. (författare)
  • Electrochemical characterization and application of azurin-modified gold electrodes for detection of superoxide
  • 2006
  • Ingår i: Biosensors & bioelectronics. - 0956-5663. ; 22:2, s. 213-219
  • Tidskriftsartikel (refereegranskat)abstract
    • A novel biosensor for superoxide radical (O2{radical dot}-) detection based on Pseudomonas aeruginosa azurin immobilized on gold electrode was designed. The rate constant of azurin reduction by O2{radical dot}- was found to be 105 M-1 s-1 in solution and five times lower, i.e., 0.2 × 105 M-1 s-1, for azurin coupled to gold by 3,3′-dithiobis(sulfosuccinimidylpropionate) (DTSSP). The electron transfer rate between the protein and the electrode ranged from 2 to 6 s-1. The sensitivity of this biosensor to O2{radical dot}- was 6.8 × 102 A m-2 M-1. The response to the interference substances, such as uric acid, H2O2, and dimethylsulfoxide was negligible below 10 μM. The electrode was applied in three O2{radical dot}- generating systems: (i) xanthine oxidase (XOD), (ii) potassium superoxide (KO2), and (iii) stimulated neutrophil granulocytes. The latter was compared with luminol-amplified chemiluminescence. The biosensor responded to O2{radical dot}- in all three environments, and the signals were antagonized by superoxide dismutase. © 2006 Elsevier B.V. All rights reserved.
  • Shleev, Sergey, et al. (författare)
  • Simultaneous use of electrochemistry and chemiluminescence to detect reactive oxygen species produced by human neutrophils
  • 2008
  • Ingår i: Cell Biology International. - 1065-6995. ; 32:12, s. 1486-1496
  • Tidskriftsartikel (refereegranskat)abstract
    • A novel approach for the simultaneous optical and electrochemical detection of biologically produced reactive oxygen species has been developed and applied. The set-up consists of a luminol-dependent chemiluminescence assay combined with two amperometric biosensors sensitive to superoxide anion radicals (O-2(center dot-))and hydrogen peroxide (H2O2), respectively. The method permits direct, real-time in vitro determination of both extra-and intracellular O-2(center dot-) and H2O2 produced by human neutrophil granulocytes. The rate of O-2(center dot-) production by stimulated neutrophils was calculated to about 10(-17) mol s(-1) per single cell. With inhibited NADPH oxidase, a distinct extracellular release of H2O2 instead of O-2(center dot-) was obtained from stimulated neutrophils with the rate of about 3 . 10(-18) mol s(-1) per single cell. When the H2O2 release was discontinued, fast H2O2 utilisation was observed. Direct interaction with and possibly attachment of neutrophils to redox protein-modified gold electrodes, resulted in a spontaneous respiratory burst in the population of cells closely associated to the electrode surface. Hence, further stimulation of human neutrophils with a potent receptor agonist (fMLF) did not significantly increase the O-2(center dot-) sensitive amperometric response. By contrast, the H2O2 sensitive biosensor, based on an HRP-modified graphite electrode, was able to reflect the bulk concentration of H2O2, produced by stimulated neutrophils and would be very useful in modestly equipped biomedical research laboratories. In summary, the system would also be appropriate for assessment of several other metabolites in different cell types, and tissues of varying complexity, with only minor electrode modifications.
  • Shleev, Sergey, et al. (författare)
  • Biological fuel cells: Divergence of opinion.
  • 2015
  • Ingår i: Bioelectrochemistry. - Elsevier. - 1878-562X. ; 106:Pt A, s. 1-2
  • Tidskriftsartikel (refereegranskat)abstract
    • Biofuel cell research has attracted considerable attention over the last decade, e.g., according to “The Web of Science” the number of publications per year in this area has been continuously increasing from 108 in 2004 to 1782 in 2014. In this short communication written in a provocative manner we, the guest editors of this Special Issue of Bioelectrochemistry devoted to biofuel cells, try to overstress the differences in current viewpoints in order to emphasize existing problems rather than just describe current opinion and praise to the skies perceived outstanding achievements, though these definitely exist in the field of bioelectrochemistry in general, and biological fuel cell research in particular. Emphasis on problems and disagreements can help push the field even further, resulting in a better understanding of the fundamental principles of the operation of biocatalysts. We do also believe that provoking the scientific community brings us much more closer to the final end point of all our research, i.e., general (large-scale) usage of “green” renewable biodevices for electric power generation and possibly even for concomitant storage of electric energy (vide infra).
  • Coman, Vasile, et al. (författare)
  • A membrane-, mediator-, cofactor-less glucose/oxygen biofuel cell.
  • 2008
  • Ingår i: Physical Chemistry Chemical Physics. - Royal Society of Chemistry. - 1463-9084. ; 10:40, s. 6093-6096
  • Tidskriftsartikel (refereegranskat)abstract
    • We report the fabrication and characterisation of a non-compartmentalised, mediator and cofactor free glucose-oxygen biofuel cell based on adsorbed enzymes exhibiting direct bioelectrocatalysis, viz. cellobiose dehydrogenase from Dichomera saubinetii and laccase from Trametes hirsuta as the anodic and cathodic bioelements, respectively, with the following characteristics: an open-circuit voltage of 0.73 V; a maximum power density of 5 muW cm(-2) at 0.5 V of the cell voltage and an estimated half-life of >38 h in air-saturated 0.1 M citrate-phosphate buffer, pH 4.5 containing 5 mM glucose.
  • Falk, Magnus, et al. (författare)
  • Biofuel cell as a power source for electronic contact lenses.
  • 2012
  • Ingår i: Biosensors & Bioelectronics. - Elsevier. - 1873-4235. ; 37:1, s. 38-45
  • Tidskriftsartikel (refereegranskat)abstract
    • Here we present unequivocal experimental proof that microscale cofactor- and membrane-less, direct electron transfer based enzymatic fuel cells do produce significant amounts of electrical energy in human lachrymal liquid (tears). 100μm diameter gold wires, covered with 17nm gold nanoparticles, were used to fashion three-dimensional nanostructured microelectrodes, which were biomodified with Corynascus thermophilus cellobiose dehydrogenase and Myrothecium verrucaria bilirubin oxidase as anodic and cathodic bioelements, respectively. The following characteristics of miniature glucose/oxygen biodevices operating in human tears were registered: 0.57V open-circuit voltage, about 1μWcm(-2) maximum power density at a cell voltage of 0.5V, and more than 20h operational half-life. Theoretical calculations regarding the maximum recoverable electrical energy can be extracted from the biofuel and the biooxidant, glucose and molecular oxygen, each readily available in human lachrymal liquid, fully support our belief that biofuel cells can be used as electrical power sources for so called smart contact lenses.
  • Falk, Magnus, et al. (författare)
  • Self-powered wireless carbohydrate/oxygen sensitive biodevice based on radio signal transmission.
  • 2014
  • Ingår i: PLoS ONE. - Public Library of Science. - 1932-6203. ; 9:10
  • Tidskriftsartikel (refereegranskat)abstract
    • Here for the first time, we detail self-contained (wireless and self-powered) biodevices with wireless signal transmission. Specifically, we demonstrate the operation of self-sustained carbohydrate and oxygen sensitive biodevices, consisting of a wireless electronic unit, radio transmitter and separate sensing bioelectrodes, supplied with electrical energy from a combined multi-enzyme fuel cell generating sufficient current at required voltage to power the electronics. A carbohydrate/oxygen enzymatic fuel cell was assembled by comparing the performance of a range of different bioelectrodes followed by selection of the most suitable, stable combination. Carbohydrates (viz. lactose for the demonstration) and oxygen were also chosen as bioanalytes, being important biomarkers, to demonstrate the operation of the self-contained biosensing device, employing enzyme-modified bioelectrodes to enable the actual sensing. A wireless electronic unit, consisting of a micropotentiostat, an energy harvesting module (voltage amplifier together with a capacitor), and a radio microchip, were designed to enable the biofuel cell to be used as a power supply for managing the sensing devices and for wireless data transmission. The electronic system used required current and voltages greater than 44 µA and 0.57 V, respectively to operate; which the biofuel cell was capable of providing, when placed in a carbohydrate and oxygen containing buffer. In addition, a USB based receiver and computer software were employed for proof-of concept tests of the developed biodevices. Operation of bench-top prototypes was demonstrated in buffers containing different concentrations of the analytes, showcasing that the variation in response of both carbohydrate and oxygen biosensors could be monitored wirelessly in real-time as analyte concentrations in buffers were changed, using only an enzymatic fuel cell as a power supply.
  • González-Arribas, Elena, et al. (författare)
  • Solar biosupercapacitor
  • 2017
  • Ingår i: Electrochemistry Communications. - Elsevier. - 1388-2481. ; 74, s. 9-13
  • Tidskriftsartikel (refereegranskat)abstract
    • Here we report on an entirely new kind of bioelectronic device – a solar biosupercapacitor, which is built from a dual-feature photobioanode combined with a double-function enzymatic cathode. The self-charging biodevice, based on transparent nanostructured indium tin oxide electrodes modified with biological catalysts, i.e. thylakoid membranes and bilirubin oxidase, is able to capacitively store electricity produced by direct conversion of radiant energy into electric energy. When self-charged during 10 min, using ambient light only, the biosupercapacitor provided a maximum of 6 mW m− 2 at 0.20 V.
  • Haberska, Karolina, et al. (författare)
  • Activity of lactoperoxidase when adsorbed on protein layers
  • 2008
  • Ingår i: Talanta. - Elsevier. - 1873-3573. ; 76:5, s. 1159-1164
  • Tidskriftsartikel (refereegranskat)abstract
    • Lactoperoxidase (LPO) is an enzyme, which is used as an antimicrobial agent in a number of applications, e.g., food technology. In the majority of applications LPO is added to a homogeneous product phase or immobilised on product surface. In the latter case, however, the measurements of LPO activity are seldom reported. In this paperwe have assessed LPO enzymatic activity on bare and protein modified gold surfaces by means of electrochemistry. It was found that LPO rapidly adsorbs to bare gold surfaces resulting in an amount of LPO adsorbed of 2.9mg/m2. A lower amount of adsorbed LPO is obtained if the gold surface is exposed to bovine serum albumin, bovine or human mucin prior to LPO adsorption. The enzymatic activity of the adsorbed enzyme is in general preserved at the experimental conditions and varies only moderately when comparing bare gold and gold surface pretreated with the selected proteins. The measurement of LPO specific activity, however, indicate that it is about 1.5 times higher if LPO is adsorbed on gold surfaces containing a small amount of preadsorbed mucin in comparison to the LPO directly adsorbed on bare gold.
  • Haberska, Karolina, et al. (författare)
  • Direct electron transfer reactions between human ceruloplasmin and electrodes.
  • 2009
  • Ingår i: Bioelectrochemistry. - Elsevier. - 1878-562X. ; 76, s. 34-41
  • Tidskriftsartikel (refereegranskat)abstract
    • In an effort to find conditions favouring bioelectrocatalytic reduction of oxygen by surface-immobilised human ceruloplasmin (Cp), direct electron transfer (DET) reactions between Cp and an extended range of surfaces were considered. Exploiting advances in surface nanotechnology, bare and carbon-nanotube-modified spectrographic graphite electrodes as well as bare, thiol- and gold-nanoparticle-modified gold electrodes were considered, and ellipsometry provided clues as to the amount and form of adsorbed Cp. DET was studied under different conditions by cyclic voltammetry and chronoamperometry. Two Faradaic processes with midpoint potentials of about 400 mV and 700 mV vs. NHE, corresponding to the redox transformation of copper sites of Cp, were clearly observed. In spite of the significant amount of Cp adsorbed on the electrode surfaces, as well as the quite fast DET reactions between the redox enzyme and electrodes, bioelectrocatalytic reduction of oxygen by immobilised Cp was never registered. The bioelectrocatalytic inertness of this complex multi-functional redox enzyme interacting with a variety of surfaces might be associated with a very complex mechanism of intramolecular electron transfer involving a kinetic trapping behaviour.
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