| 11. |
- Saharil, Farizah, 1976-, et al.
(author)
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SUPERIOR DRY BONDING OF OFF-STOICHIOMETRY THIOL-ENEEPOXY (OSTE(+)) POLYMERS FOR HETEROGENEOUS MATERIALLABS-ON-CHIP
- 2012
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In: Proceedings Micro Total Analysis Systems (muTAS) 2012. - 9780979806452 ; , s. 1831-1833
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Conference paper (peer-reviewed)abstract
- We demonstrate biocompatible bonding to a multitude of LoC substrates using OSTE(+), a novel polymer formulation with a unique dual cure system developed specifically for microfluidic applications. OSTE(+) allows for soft lithography microstructuring, strong biocompatible dry bonding to almost any substrate during LoCmanufacturing, while simultaneously mimicking the mechanical properties found in thermoplastic polymers, hence allowing for true prototyping of commercial LoCs. In this work, we describe the simple micropatterning process, curing mechanisms and show leak-free dry bonding to nine different substrate materials. Uniquely, strong bonding is achieved to COC, the thermoplastic of choice for microfluidics, which opens up the possibility of hybrid thermoplastic/OSTE(+) LoCs. In this work, we demonstrate bonding to nine different untreated substrates at 37°C and room temperature to demonstrate good bonding properties even at lower temperature. We also show the high resilience of the epoxy bond by immersing an aluminum substrate with a bonded OSTE(+) layer into boiling water while simultaneously subjecting the assembly to pressurized air.
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| 12. |
- Salm, E., et al.
(author)
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Integrated 'lab-on-a-transistor' : With droplets-in-air for parallel nanoliter reactions
- 2012
-
In: Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. - : Chemical and Biological Microsystems Society. - 9780979806452 ; , s. 1945-1947
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Conference paper (peer-reviewed)abstract
- We aim to take 'lab-on-chip' technology further by introducing the concept of a 'lab-on-transistor'. In this methodology, laboratory operations, such as heating, cell lysis, and detection, are performed on a single transistor instead of on an entire microchip. To demonstrate this concept, we developed a heating technique that allows transistors to act as electrically addressable, individual heating units. We have coupled the transistor heaters with placement of sub-nanoliter droplets to create individual heated reaction volumes. Under this configuration transistors become highly localized heater/sensors capable of high-speed thermocycling (>25°C/s) of <1nL reactions with potential for electrical detection of biological analytes.
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| 13. |
- Sjöström, Staffan L., et al.
(author)
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Multiplex analysis of enzyme kinetics and inhibition by droplet microfluidics using picoinjectors
- 2012
-
In: Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. - 9780979806452 ; , s. 172-174
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Conference paper (peer-reviewed)abstract
- We present a novel microdroplet-based device for extensive characterization of the reaction kinetics of enzymeinhibitor systems in a single experiment, for the first time utilizing droplet picoinjectors for bioanalysis. This device enables the scanning of multiple inhibitors, inhibitor concentrations and substrate conditions in a single, highly time resolved experiment yielding the Michaelis constant (Km), the turnover number (Kcat) the mode of inhibition and the inhibitor enzyme binding constants (Ki, Ki). Using this device we determine Km and Kcat for β-galactosidase and the fluorogenic substrate Resorufin β-D-galactopyranoside (RBG) to 252 μM and 477 s-1, respectively. Furthermore, we examine the inhibitory effects of Phenylethyl β-D-thiogalactopyranoside (PETG) on this system.
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| 14. |
- Weibull, Emilie, et al.
(author)
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Development of a microfluidic concentration gradient generator on a microwell slide for high-throughput cell analysis
- 2012
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In: Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. - : Chemical and Biological Microsystems Society. - 9780979806452 ; , s. 1573-1575
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Conference paper (peer-reviewed)abstract
- The present study reports a newly developed, microfluidic concentration gradient generator that adds substantial utility to the high-throughput nature of the microwell slide that we have previously established [1, 2]. Using microfluidic dynamics, we have designed microchannels to deliver a reagent and its dilutant with precisely controlled flow volumes, generating 8 discrete steps of reagent concentration in designated microwells. As proof of concept FITC-labeled dextran and dextran-free water was used to visualize the concentration gradient. The next step will be to perform cancer drug concentration gradient experiments on an animal cell line.
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