| 1. |
- Larsson, P A, et al.
(författare)
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Non-random chromosome rearrangements in herpes simplex virus type 1 transformed diploid CHEF cells
- 1992
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Ingår i: Anticancer Research. - 0250-7005 .- 1791-7530. ; 12:3, s. 863-868
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Tidskriftsartikel (refereegranskat)abstract
- The effects of Herpes simplex virus type 1 (HSV-1) on diploid, non-tumourigenic Chinese hamster embryo fibroblasts (CHEF/18-1D-3) were studied. Six independent lines transformed by HSV-1 alone or by HSV-1 in combination with acyclovir or aqueous tobacco extract were isolated. In contrast to uninfected CHEF/18-1D-3 cells, all transformants grew in soft agar and were tumourigenic in nude mice. Neither infectious virus nor viral antigens could be detected in any of the lines. Cytogenetic analysis revealed clonal chromosome abnormalities in all lines including trisomy for the long arm of chromosome 3 in five lines. In three of these the extra 3q was translocated onto 6p. All lines showed loss of the corresponding 3p arm. The remaining line had a hypodiploid stemline with loss of one chromosome 7. This line also showed a pronounced chromosomal instability with a multitude of mainly sporadic rearrangements. These results show that HSV-1 induced transformation and tumourigenesis in CHEF cells is associated with the induction of chromosome rearrangements, in particular trisomy for 3q.
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| 2. |
- Westerdahl, J., et al.
(författare)
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Southern travelling habits with special reference to tumour site in Swedish melanoma patients
- 1992
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Ingår i: Anticancer research. - 0250-7005. ; 12:5, s. 1539-1542
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Tidskriftsartikel (refereegranskat)abstract
- Southern travelling habits were recorded for 127 melanoma patients from southern parts of Sweden (the 56th latitude), 55 thyroid cancer patients, 100 non-Hodgkin's patients and 794 healthy controls from the same region. Melanoma patients were found to travel significantly more often south of the 45th latitude, as compared with patients with non-Hodgkin's lymphoma or thyroid carcinoma (RR = 2.2 for a difference of + 10 trips), and with the healthy controls (RR = 1.4 for a difference of + 10 trips). Considering men and women sepatately, the difference was significant only for men. Patients with melanoma had a higher educational level than the tumour controls and the healthy controls (p<0.001 and p<0.001 respectively). There was a significant correlation between high travelling frequency and high education. An increased risk related to southern travelling was present for patients with melanoma on the extremities and head and neck, as well as for patients with truncal melanoma. These findings support the concept that acute exposure to sunburn may be a risk factor for malignant melanoma.
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