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Träfflista för sökning "WFRF:(Holmgren E) ;conttype:(scientificother)"

Search: WFRF:(Holmgren E) > Other academic/artistic

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  • FitzGerald, Edward A., et al. (author)
  • Multiplexed experimental strategies for fragment library screening using SPR biosensors
  • 2020
  • Other publication (other academic/artistic)abstract
    • Surface plasmon resonance biosensor technology (SPR) is ideally suited for fragment-based lead discovery. However, generally suitable experimental procedures or detailed protocols are lacking, especially for structurally or physico-chemically challenging targets or when tool compounds are lacking. Success depends on accounting for the features of both the target and the chemical library, purposely designing screening experiments for identification and validation of hits with desired specificity and mode-of-action, and availability of orthogonal methods capable of confirming fragment hits. By adopting a multiplexed strategy, the range of targets and libraries amenable to an SPR biosensor-based approach for identifying hits is considerably expanded. We here illustrate innovative strategies using five challenging targets and variants thereof. Two libraries of 90 and 1056 fragments were screened using two different flow-based SPR biosensor systems, allowing different experimental approaches. Practical considerations and procedures accounting for the characteristics of the proteins and libraries, and that increase robustness, sensitivity, throughput and versatility are highlighted.Competing Interest StatementAnna Moberg, Maria T. Lindgren and Claes Holmgren work for Cytiva, which produce Biacore systems.
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  • Result 1-10 of 39
Type of publication
conference paper (16)
journal article (11)
other publication (5)
book chapter (4)
reports (1)
doctoral thesis (1)
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licentiate thesis (1)
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Type of content
Author/Editor
Holmgren, A (10)
Holmgren, E. (10)
Gronlund, H (8)
Gafvelin, G (8)
Olsson, T (7)
Bronge, M (7)
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Nilsson, O (6)
Martin, R (5)
Thomas, O (5)
Khademi, M. (4)
Holmgren, Johan (3)
Ruhrmann, S (3)
Lundgren, E. (3)
Kaiser, A (3)
Holmgren, G (3)
Ulander, Lars, 1962 (3)
Berndt, Kurt D (3)
Arner, E. S. J. (3)
Hellström, Cecilia (2)
Nilsson, Peter (2)
Kockum, I. (2)
Abramsson, Mia (2)
Näslund, Ulf (2)
Danielson, U. Helena (2)
Holmgren, Anders (2)
Gräslund, Torbjörn (2)
Akpinar, B (2)
Hamilton, David J. (2)
Drugge, Ulf (2)
Steen, L (2)
Holmes, E. (2)
Brundin, L (2)
Moberg, Anna (2)
Fransson, Johan E.S. (2)
Fransson, J E S (2)
Henein, Michael (2)
Sandgren, O (2)
Mishra, P (2)
Carvalho-Queiroz, C (2)
Coppo, L (2)
O'Brien, Peter (2)
Carvalho-Querioz, C. (2)
Sengupta, R (2)
Gräslund, Torbjörn, ... (2)
Sandberg, Gustaf, 19 ... (2)
Vagrys, Darius (2)
Opassi, Giulia (2)
Klein, Hanna F. (2)
Talibov, Vladimir O. (2)
Davis, Ben (2)
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University
Karolinska Institutet (20)
Royal Institute of Technology (4)
Uppsala University (4)
Södertörn University (3)
Chalmers University of Technology (3)
Umeå University (2)
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Mälardalen University (2)
Linnaeus University (2)
Swedish University of Agricultural Sciences (2)
University of Gothenburg (1)
University of Gävle (1)
Linköping University (1)
Red Cross University College (1)
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Language
English (37)
Swedish (1)
Undefined language (1)
Research subject (UKÄ/SCB)
Natural sciences (6)
Medical and Health Sciences (6)
Engineering and Technology (3)
Agricultural Sciences (3)
Social Sciences (3)

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