| 1. |
|
|
| 2. |
- Holgersson, Jan, et al.
(author)
-
A case of acute vascular rejection caused by endothelial-reactive non-HLA antibodies.
- 2007
-
In: Clinical transplants. - 0890-9016. ; , s. 535-8
-
Journal article (other academic/artistic)abstract
- We describe a female patient who, despite negative conventional cross-matches, lost her first kidney graft in an acute humoral rejection. Prior to the second, AB0-incompatible (A1B to A1) living-donor kidney transplant, the patient had negative T- and B-cell cross-matches but had a positive donor-reactive endothelial cell cross-match. Following pre-transplant protein A and GlycoSorb-ABO immunoadsorptions to remove blood group B and anti-endothelial cell antibodies, Mabthera, and IVIG administrations, she was successfully transplanted. By the second post-operative day, creatinine levels were down to 96 micromol/L from 611 micromol/L pre-operatively. On day 9 creatinine rose again, and on the same day the endothelial cell crossmatch became positive for IgG, whereas the T-cell cross-match remained negative and the anti-A1B titers remained low. A kidney biopsy taken on day 10 post-transplant showed a picture of an acute vascular, antibody-mediated rejection. Following rejection treatment and repeated protein A and Glyco-Sorb-ABO immunoadsorptions, the patient's kidney function was again normalized. The use of a recently developed kit (XM-ONE) for the detection of anti-endothelial cell antibodies allowed us to identify a patient at risk for developing acute antibody-mediated rejection as well as to monitor treatment efficacy and post-transplant complications.
|
|
| 3. |
- Nygren, Yvonne, 1965-
(author)
-
Advances in analytical methodologies for studies of the platinum metallome in malignant cells exposed to cisplatin
- 2010
-
Doctoral thesis (other academic/artistic)abstract
- The scientific progress about the important chemotherapeutic drug substance cisplatin (CDDP) and its function has often been rendered by data difficult to interpret, and still many questions about its mode of action remains to be clarified by the scientific community. However, studies of CDDP possess a high complexity due to; i) low intracellular concentration, ii) many potential biomolecule targets, iii) poor or unknown stability of the intact drug and its biomolecule adducts and iv) complex and varying sample matrices. Metallomic studies, using advanced analytical techniques may contribute to clarify the interactions between CDDP and intracellular biomolecules. For a successful outcome sample preparation conditions as well as separation and detection techniques must be carefully selected and optimized to achieve accurate results and correct interpretation of data. This thesis describes some new and improved analytical methodologies for characterizing the Pt metallome in CDDP-exposed malignant cells. The developed methods are based on powerful liquid chromatography (LC) methods hyphenated to sensitive detection by inductively coupled plasma- (ICP) and electrospray ionization mass spectrometry (ESIMS). Consideration has also been taken about sample preparation conditions. By selecting “chemically inert” sample preparation (cell lysis by osmosis) and separation (using only nonreactive or no additatives) conditions we could avoid the formation of platinum artifact compounds previously described in the literature (Paper I and II). Using oxygen containing organic solvents with high boiling points (dimethylformamide; DMF, 1,4-dioxane, n-propanol and ethanol) as alternatives to acetonitrile in the LC separations, significant improvements were achieved in ICPMS sensitivity and robustness. When evaluated in combination with chromatographic performance and ESIMS detection the overall best performance was achieved with n-propanol (Paper II, III and IV). From the studies in Paper II we could show that free intact CDDP can be found in malignant cells, as supporting evidence for passive or endocytotic uptake of the drug and further estimate a half-life for intracellular CDDP to about 15 minutes. Such data has not been shown before. In Paper V, the above improved LC methods were used to demonstrate differences in the platinum and cupper metallome from sensitive and resistant T289 melanoma cells exposed to CDDP at near clinical levels. In a wider perspective we have shown the potential of using hydrophilic liquid interaction chromatography (HILIC) hyphenated to ICPMS detection as a general approach for analysis of hydrophilic metallo-compounds (Paper II). Taking advantage of the superior ICPMS performance using n-propanol gradients for reversed phase liquid chromatography (RPLC) possess a true alternative and /or complimentary technique to size exclusion chromatography (SEC) commonly applied within metallomic studies of biomolecules (Paper V). Using n-propanol in HILIC as well as in RPLC enables parallel detection by ICP- and ESIMS using only one set of chromatographic parameters (Paper III and IV), something commonly called for by scientists in the field.
|
|
| 4. |
|
|
| 5. |
- Cerna, Katarina, et al.
(author)
-
Nurses' work practices in design : managing the complexity of pain
- 2020
-
In: Journal of Workplace Learning. - : Emerald Group Publishing Limited. - 1366-5626 .- 1758-7859. ; 32:2, s. 135-146
-
Journal article (peer-reviewed)abstract
- Purpose: The purpose of this study is to understand the activities in nurses' work practices in relation to the design process of a self-monitoring application. Design/methodology/approach: A design ethnographic approach was applied in this study. Findings: To solve the problem of translating highly qualitative phenomena, such as pain, into the particular abstract features of a self-monitoring application, design participants had to balance these two aspects by managing complexity. In turn, the nurses'€™ work practices have changed because it now involves a new activity based on a different logic than the nurses’ traditional work practices. Originality/value: This study describes a new activity included in nurses’ work practices when the nurses became part of a design process. This study introduces a novel way on how to gain a deeper understanding of existing professional practice through a detailed study of activities taking place in a design process. This study explores the possible implications for nurses’ professional practices when they participate in a self-monitoring application design process. Â2020, Emerald Publishing Limited.
|
|
| 6. |
- Elgan, Elisabeth
(author)
-
Le printemps 1968 en Suède
- 1988
-
In: Mai 68. - Nanterre : BDIC. - 2707117676 ; , s. 56-59
-
Book chapter (other academic/artistic)
|
|
| 7. |
|
|
| 8. |
- Frid, Emma
(author)
-
Sonification of women in sound and music computing - The sound of female authorship in ICMC, SMC and NIME proceedings
- 2017
-
In: 2017 ICMC/EMW - 43rd International Computer Music Conference and the 6th International Electronic Music Week. - : Shanghai Conservatory of Music. - 9780984527465 ; , s. 233-238
-
Conference paper (peer-reviewed)abstract
- The primary goal of this study was to approximate the number of female authors in the academic field of Sound and Music Computing. This was done through gender prediction from author names for proceedings from the ICMC, SMC and NIME conferences, and by sonifying these results. Although gender classification by first name can only serve as an estimation of the actual number of female authors in the field, some conclusions could be drawn. The total percentage of author names classified as female was 10.3% for ICMC, 11.9% for SMC and 11.9% for NIME. When merging data from all three conferences for years 2004-2016, it could be concluded that names classified as female ranged from 9.5 to 14.3%. Changes in the ratio of female vs. male authors over time were further illustrated by sonifications, allowing the reader to explore, compare and reflect upon the results by listening to sonic representations of the data. The conclusion that can be drawn from this study is that the field of Sound and Music Computing is still far from being gender-balanced.
|
|
| 9. |
|
|
| 10. |
|
|
