Search: onr:"swepub:oai:DiVA.org:su-141400" > Epitopes of anti-RI...
Fältnamn | Indikatorer | Metadata |
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000 | 04395naa a2200565 4500 | |
001 | oai:DiVA.org:su-141400 | |
003 | SwePub | |
008 | 170421s2017 | |||||||||||000 ||eng| | |
009 | oai:DiVA.org:kth-204077 | |
009 | oai:prod.swepub.kib.ki.se:135226043 | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-1414002 URI |
024 | 7 | a https://doi.org/10.1038/srep431902 DOI |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-2040772 URI |
024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:1352260432 URI |
040 | a (SwePub)sud (SwePub)kthd (SwePub)ki | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Ch'ng, Jun-Hongu Karolinska Institutet4 aut |
245 | 1 0 | a Epitopes of anti-RIFIN antibodies and characterization of rif-expressing Plasmodium falciparum parasites by RNA sequencing |
264 | c 2017-02-24 | |
264 | 1 | b Springer Science and Business Media LLC,c 2017 |
338 | a print2 rdacarrier | |
500 | a QC 20170329 | |
520 | a Variable surface antigens of Plasmodium falciparum have been a major research focus since they facilitate parasite sequestration and give rise to deadly malaria complications. Coupled with its potential use as a vaccine candidate, the recent suggestion that the repetitive interspersed families of polypeptides (RIFINs) mediate blood group A rosetting and influence blood group distribution has raised the research profile of these adhesins. Nevertheless, detailed investigations into the functions of this highly diverse multigene family remain hampered by the limited number of validated reagents. In this study, we assess the specificities of three promising polyclonal anti-RIFIN antibodies that were IgG-purified from sera of immunized animals. Their epitope regions were mapped using a 175,000-peptide microarray holding overlapping peptides of the P. falciparum variable surface antigens. Through immunoblotting and immunofluorescence imaging, we show that different antibodies give varying results in different applications/assays. Finally, we authenticate the antibody-based detection of RIFINs in two previously uncharacterized non-rosetting parasite lines by identifying the dominant rif transcripts using RNA sequencing. | |
650 | 7 | a NATURVETENSKAPx Biologi0 (SwePub)1062 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciences0 (SwePub)1062 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Immunologi inom det medicinska området0 (SwePub)301102 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Immunology in the medical area0 (SwePub)301102 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaper0 (SwePub)3012 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicine0 (SwePub)3012 hsv//eng |
700 | 1 | a Sirel, Madleu Karolinska Institutet4 aut |
700 | 1 | a Zandian, Arashu KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u11qiehv |
700 | 1 | a del Pilar Quintana, Maria4 aut |
700 | 1 | a Chan, Sherwin Chun Leung4 aut |
700 | 1 | a Moll, Kirstenu Karolinska Institutet4 aut |
700 | 1 | a Tellgren-Roth, Åsau Stockholms universitet,Institutionen för biokemi och biofysik4 aut0 (Swepub:su)aste1633 |
700 | 1 | a Nilsson, IngMarieu Stockholms universitet,Institutionen för biokemi och biofysik4 aut0 (Swepub:su)inils |
700 | 1 | a Nilsson, Peteru KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u1ws88sk |
700 | 1 | a Qundos, Ulrikau KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u1m6ivho |
700 | 1 | a Wahlgren, Matsu Karolinska Institutet4 aut |
710 | 2 | a Karolinska Institutetb Proteomik och nanobioteknologi4 org |
773 | 0 | t Scientific Reportsd : Springer Science and Business Media LLCg 7q 7x 2045-2322 |
856 | 4 | u https://doi.org/10.1038/srep43190y Fulltext |
856 | 4 | u https://www.nature.com/articles/srep43190.pdf |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-141400 |
856 | 4 8 | u https://doi.org/10.1038/srep43190 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-204077 |
856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:135226043 |
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