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Cytokine and antibo...
Cytokine and antibody responses in birch-pollen-allergic patients treated with genetically modified derivatives of the major birch pollen allergen Bet v 1
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- Gafvelin, G (author)
- Karolinska Institutet
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- Thunberg, Sarah, 1976- (author)
- Karolinska Institutet
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- Kronqvist, M (author)
- Karolinska institutet
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- Gronlund, H (author)
- Karolinska Institutet
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- Gronneberg, R (author)
- Karolinska Institutet
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- Troye-Blomberg, M (author)
- Karolinska institutet
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- Akdis, M (author)
- SIAF, switzerland
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- Fiebig, H (author)
- Allergopharma, Germany
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- Purohit, A (author)
- Hôpitaux Universitaires de Strasbourg, France
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- Horak, F (author)
- Medical University of Vienna, Austria
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- Reisinger, J (author)
- Medical University of Vienna, Austria
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- Niederberger, V (author)
- Medical University of Vienna, Austria
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- Akdis, C A (author)
- SIAF Switzerland
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- Cromwell, O (author)
- Allergopharma, Germany
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- Pauli, G (author)
- Hôpitaux Universitaires de Strasbourg, France
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- Valenta, R (author)
- Medical University of Vienna, Austria
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- van Hage, M (author)
- Karolinska Institutet
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(creator_code:org_t)
- 2005-09-16
- 2005
- English.
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In: International Archives of Allergy and Immunology. - : S. Karger AG. - 1018-2438 .- 1423-0097. ; 138:1, s. 59-66
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Background: Recently, recombinant hypoallergenic derivatives of the major birch pollen allergen, Bet v 1, were used to treat birch-pollen-allergic patients in a double-blind, placebo-controlled, multi-centre immunotherapy study. The aim of this study was to evaluate the effects of vaccination with aluminium-hydroxide-adsorbed recombinant Bet v 1 derivatives versus placebo on T-cell, cytokine and antibody responses in a subgroup of patients. Methods: Blood was drawn from patients of the Swedish centre (n=27; rBet v 1 fragments: n=10; rBet v 1 trimer: n=8, and placebo-aluminium hydroxide: n=9) before the start and after completion of the treatment. PBMC were stimulated with rBet v 1 and analysed for cytokine (IL-4, IL-5, IL-10, IL-12, IL-13 and IFN-gamma)-secreting cells by ELISpot. Bet v 1-specific antibody levels in serum (IgG(1-4), IgE and IgA) were measured by ELISA. Skin prick tests with defined Bet v 1 concentrations were performed before and 10-11 months after the beginning of the study. Results: Bet v 1-specific IgG levels, consisting of IgG 1, IgG 2 and IgG 4, were significantly increased after treatment with recombinant allergen derivatives. Treatment with rBet v 1 trimer led to a significant (p<0.05) reduction of Bet v 1-reactive IL-5- and IL-13-producing cells, reflecting a reduced Th2 response. In addition, a decreased number of Bet v 1-reactive IL-4 producing (p=0.07) and an increase of IL-12-producing (p=0.06) cells was noted in the trimer-treated patients. In contrast to placebo, active treatment resulted in significantly reduced immediate-type skin reactions to Bet v 1 even 10-11 months after treatment. Conclusion: Vaccination with recombinant hypoallergenic Bet v 1 derivatives induces a Bet v 1-specific IgG response and leads to reduced skin reactivity in allergic patients. A reduction of Bet v 1-specific Th2 responses was observed in trimer-treated patients, which may reflect the intrinsic property of this allergen derivative. Copyright (C) 2005 S. Karger AG, Basel.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
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- ref (subject category)
- art (subject category)
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- By the author/editor
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Gafvelin, G
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Thunberg, Sarah, ...
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Kronqvist, M
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Gronlund, H
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Gronneberg, R
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Troye-Blomberg, ...
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show more...
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Akdis, M
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Fiebig, H
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Purohit, A
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Horak, F
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Reisinger, J
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Niederberger, V
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Akdis, C A
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Cromwell, O
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Pauli, G
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Valenta, R
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van Hage, M
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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International Ar ...
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Royal Institute of Technology
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Karolinska Institutet