Search: onr:"swepub:oai:DiVA.org:kth-75531" > Imaging of Human Ep...
Fältnamn | Indikatorer | Metadata |
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000 | 04782naa a2200505 4500 | |
001 | oai:DiVA.org:kth-75531 | |
003 | SwePub | |
008 | 120206s2012 | |||||||||||000 ||eng| | |
009 | oai:DiVA.org:uu-164421 | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-755312 URI |
024 | 7 | a https://doi.org/10.2967/jnumed.111.0930472 DOI |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-1644212 URI |
040 | a (SwePub)kthd (SwePub)uu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Heskamp, Sandra4 aut |
245 | 1 0 | a Imaging of Human Epidermal Growth Factor Receptor Type 2 Expression with (18)F-Labeled Affibody Molecule Z(HER2:2395) in a Mouse Model for Ovarian Cancer |
264 | c 2011-12-15 | |
264 | 1 | b Society of Nuclear Medicine,c 2012 |
338 | a print2 rdacarrier | |
500 | a QC 20120206 | |
520 | a Affibody molecules are small (7 kDa) proteins with subnanomolar targeting affinity. Previous SPECT studies in xenografts have shown that the Affibody molecule (111)In-DOTA-Z(HER2:2395) can discriminate between high and low human epidermal growth factor receptor type 2 (HER2)-expressing tumors, indicating that radiolabeled Affibody molecules have potential for patient selection for HER2-targeted therapy. Compared with SPECT, PET with positron-emitting radionuclides, such as (18)F, may improve imaging of HER2 expression because of higher sensitivity and improved quantification of PET. The aim of the present study was to determine whether the (18)F-labeled NOTA-conjugated Affibody molecule Z(HER2:2395) is a suitable agent for imaging of HER2 expression. The tumor-targeting properties of (18)F-labeled Z(HER2:2395) were compared with (111)In- and (68)Ga-labeled Z(HER2:2395) in mice with HER2-expressing SK-OV-3 xenografts. Methods: Z(HER2:2395) was conjugated with NOTA and radiolabeled with (18)F, (68)Ga, and (111)In. Radiolabeling with (18)F was based on the complexation of Al(18)F by NOTA. The 50% inhibitory concentration values for NOTA-Z(HER2:2395) labeled with (19)F, (69)Ga, and (115)In were determined in a competitive cell-binding assay using SK-OV-3 cells. Mice bearing subcutaneous SK-OV-3 xenografts were injected intravenously with radiolabeled NOTA-Z(HER2:2395). One and 4 h after injection, PET/CT or SPECT/CT images were acquired, and the biodistribution was determined by ex vivo measurement. Results: The 50% inhibitory concentration values for (19)F-, (69)Ga-, and (115)In-NOTA-Z(HER2:2395) were 5.0, 6.3, and 5.3 nM, respectively. One hour after injection, tumor uptake was 4.4 +/- 0.8 percentage injected dose per gram (% ID/g), 5.6 +/- 1.6 % ID/g, and 7.1 +/- 1.4 % ID/g for (18)F-, (68)Ga-, and (111)In-NOTA-Z(HER2:2395), respectively, and the respective tumor-to-blood ratios were 7.4 +/- 1.8, 8.0 +/- 1.3, and 4.8 +/- 1.3. Tumor uptake was specific, because uptake could be blocked efficiently by coinjection of an excess of unlabeled Z(HER2:2395). PET/CT and SPECT/CT images clearly visualized HER2-expressing SK-OV-3 xenografts. Conclusion: This study showed that (18)F-NOTA-Z(HER2:2395) is a promising new imaging agent for HER2 expression in tumors. Affibody molecules were successfully labeled with (18)F within 30 min, based on the complexation of Al(18)F by NOTA. Further research is needed to determine whether this technique can be used for patient selection for HER2-targeted therapy. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Radiologi och bildbehandling0 (SwePub)302082 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Radiology, Nuclear Medicine and Medical Imaging0 (SwePub)302082 hsv//eng |
653 | a HER2 | |
653 | a Affibody molecule | |
653 | a PET | |
653 | a (18)F | |
653 | a ovarian cancer | |
700 | 1 | a Laverman, Peter4 aut |
700 | 1 | a Rosik, Danielu KTH,Molekylär Bioteknologi4 aut0 (Swepub:kth)u1m2u8ne |
700 | 1 | a Boschetti, Frederic4 aut |
700 | 1 | a van der Graaf, Winette T. A.4 aut |
700 | 1 | a Oyen, Wim J. G.4 aut |
700 | 1 | a van Laarhoven, Hanneke W. M.4 aut |
700 | 1 | a Tolmachev, Vladimiru Uppsala universitet,Enheten för biomedicinsk strålningsvetenskap,Vladimir Tolmachev4 aut0 (Swepub:uu)vladtolm |
700 | 1 | a Boerman, Otto C.4 aut |
710 | 2 | a KTHb Molekylär Bioteknologi4 org |
773 | 0 | t Journal of Nuclear Medicined : Society of Nuclear Medicineg 53:1, s. 146-153q 53:1<146-153x 0161-5505x 1535-5667x 2159-662X |
856 | 4 | u http://jnm.snmjournals.org/content/53/1/146.full.pdf |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-75531 |
856 | 4 8 | u https://doi.org/10.2967/jnumed.111.093047 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-164421 |
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