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Up-regulation of sm...
Up-regulation of small intestinal interleukin-17 immunity in untreated coeliac disease but not in potential coeliac disease or in type 1 diabetes
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- Lahdenperä, Anna (author)
- Linköpings universitet,Pediatrik,Hälsouniversitetet
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- Hölttä, V (author)
- National Institute for Health and Welfare, Finland
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- Ruohtula, T (author)
- National Institute for Health and Welfare, Finland
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- Salo, H M (author)
- National Institute for Health and Welfare, Finland
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- Orivuori, L (author)
- National Institute for Health and Welfare, Finland
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- Westerholm-Ormio, M (author)
- Hospital for Children and Adolescents, University of Helsinki,
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- Savilahti, E (author)
- Hospital for Children and Adolescents, University of Helsinki,
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- Fälth-Magnusson, Karin (author)
- Östergötlands Läns Landsting,Linköpings universitet,Pediatrik,Hälsouniversitetet,Barn- och ungdomskliniken i Linköping
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- Högberg, Lotta (author)
- Östergötlands Läns Landsting,Linköpings universitet,Pediatrik,Hälsouniversitetet,Barn- och ungdomskliniken i Norrköping
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- Ludvigsson, Johnny (author)
- Östergötlands Läns Landsting,Linköpings universitet,Pediatrik,Hälsouniversitetet,Barn- och ungdomskliniken i Linköping
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- Vaarala, O (author)
- National Institute for Health and Welfare, Finland
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(creator_code:org_t)
- 2012-01-11
- 2012
- English.
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In: Clinical and Experimental Immunology. - : John Wiley & Sons. - 0009-9104 .- 1365-2249. ; 167:2, s. 226-234
- Related links:
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https://www.ncbi.nlm...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Up-regulation of interleukin (IL)-17 in small intestinal mucosa has been reported in coeliac disease (CD) and in peripheral blood in type 1 diabetes (T1D). We explored mucosal IL-17 immunity in different stages of CD, including transglutaminase antibody (TGA)-positive children with potential CD, children with untreated and gluten-free diet-treated CD and in children with T1D. Immunohistochemistry was used for identification of IL-17 and forkhead box protein 3 (FoxP3)-positive cells and quantitative polymerase chain reaction (qPCR) for IL-17, FoxP3, retinoic acid-related orphan receptor (ROR)c and interferon (IFN)-γ transcripts. IL-1β, IL-6 and IL-17 were studied in supernatants from biopsy cultures. Expression of the apoptotic markers BAX and bcl-2 was evaluated in IL-17-stimulated CaCo-2 cells. The mucosal expression of IL-17 and FoxP3 transcripts were elevated in individuals with untreated CD when compared with the TGA-negative reference children, children with potential CD or gluten-free diet-treated children with CD (P andlt; 0·005 for all IL-17 comparisons and P andlt; 0·01 for all FoxP3 comparisons). The numbers of IL-17-positive cells were higher in lamina propria in children with CD than in children with T1D (P andlt; 0·05). In biopsy specimens from patients with untreated CD, enhanced spontaneous secretion of IL-1β, IL-6 and IL-17 was seen. Activation of anti-apoptotic bcl-2 in IL-17-treated CaCo-2 epithelial cells suggests that IL-17 might be involved in mucosal protection. Up-regulation of IL-17 could, however, serve as a biomarker for the development of villous atrophy and active CD.
Keyword
- MEDICINE
- MEDICIN
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- ref (subject category)
- art (subject category)
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- By the author/editor
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Lahdenperä, Anna
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Hölttä, V
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Ruohtula, T
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Salo, H M
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Orivuori, L
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Westerholm-Ormio ...
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show more...
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Savilahti, E
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Fälth-Magnusson, ...
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Högberg, Lotta
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Ludvigsson, John ...
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Vaarala, O
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show less...
- Articles in the publication
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Clinical and Exp ...
- By the university
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Linköping University