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Search: onr:"swepub:oai:DiVA.org:su-141400" > Epitopes of anti-RI...

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LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004395naa a2200565 4500
001oai:DiVA.org:su-141400
003SwePub
008170421s2017 | |||||||||||000 ||eng|
009oai:DiVA.org:kth-204077
009oai:prod.swepub.kib.ki.se:135226043
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-1414002 URI
0247 a https://doi.org/10.1038/srep431902 DOI
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-2040772 URI
0247 a http://kipublications.ki.se/Default.aspx?queryparsed=id:1352260432 URI
040 a (SwePub)sud (SwePub)kthd (SwePub)ki
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Ch'ng, Jun-Hongu Karolinska Institutet4 aut
2451 0a Epitopes of anti-RIFIN antibodies and characterization of rif-expressing Plasmodium falciparum parasites by RNA sequencing
264 c 2017-02-24
264 1b Springer Science and Business Media LLC,c 2017
338 a print2 rdacarrier
500 a QC 20170329
520 a Variable surface antigens of Plasmodium falciparum have been a major research focus since they facilitate parasite sequestration and give rise to deadly malaria complications. Coupled with its potential use as a vaccine candidate, the recent suggestion that the repetitive interspersed families of polypeptides (RIFINs) mediate blood group A rosetting and influence blood group distribution has raised the research profile of these adhesins. Nevertheless, detailed investigations into the functions of this highly diverse multigene family remain hampered by the limited number of validated reagents. In this study, we assess the specificities of three promising polyclonal anti-RIFIN antibodies that were IgG-purified from sera of immunized animals. Their epitope regions were mapped using a 175,000-peptide microarray holding overlapping peptides of the P. falciparum variable surface antigens. Through immunoblotting and immunofluorescence imaging, we show that different antibodies give varying results in different applications/assays. Finally, we authenticate the antibody-based detection of RIFINs in two previously uncharacterized non-rosetting parasite lines by identifying the dominant rif transcripts using RNA sequencing.
650 7a NATURVETENSKAPx Biologi0 (SwePub)1062 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciences0 (SwePub)1062 hsv//eng
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Immunologi inom det medicinska området0 (SwePub)301102 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Immunology in the medical area0 (SwePub)301102 hsv//eng
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaper0 (SwePub)3012 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Basic Medicine0 (SwePub)3012 hsv//eng
7001 a Sirel, Madleu Karolinska Institutet4 aut
7001 a Zandian, Arashu KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u11qiehv
7001 a del Pilar Quintana, Maria4 aut
7001 a Chan, Sherwin Chun Leung4 aut
7001 a Moll, Kirstenu Karolinska Institutet4 aut
7001 a Tellgren-Roth, Åsau Stockholms universitet,Institutionen för biokemi och biofysik4 aut0 (Swepub:su)aste1633
7001 a Nilsson, IngMarieu Stockholms universitet,Institutionen för biokemi och biofysik4 aut0 (Swepub:su)inils
7001 a Nilsson, Peteru KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u1ws88sk
7001 a Qundos, Ulrikau KTH,Proteomik och nanobioteknologi,Science for Life Laboratory, SciLifeLab4 aut0 (Swepub:kth)u1m6ivho
7001 a Wahlgren, Matsu Karolinska Institutet4 aut
7102 a Karolinska Institutetb Proteomik och nanobioteknologi4 org
7730 t Scientific Reportsd : Springer Science and Business Media LLCg 7q 7x 2045-2322
8564 u https://doi.org/10.1038/srep43190y Fulltext
8564 u https://www.nature.com/articles/srep43190.pdf
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-141400
8564 8u https://doi.org/10.1038/srep43190
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-204077
8564 8u http://kipublications.ki.se/Default.aspx?queryparsed=id:135226043

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