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Overexpression of v...
Overexpression of vesicle-associated membrane protein PttVAP27-17 as a tool to improve biomass production and the overall saccharification yields in Populus trees
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- Gandla, Madhavi Latha (author)
- Umeå universitet,Kemiska institutionen
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- Mähler, Niklas (author)
- Umeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC)
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- Escamez, Sacha, 1987- (author)
- Umeå universitet,Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology,Umeå University,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC),Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Sweden
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- Skotare, Tomas (author)
- Umeå universitet,Kemiska institutionen
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- Obudulu, Ogonna, 1976 (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology,Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden
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- Moller, L. (author)
- SweTree Technologies, Umeå, Sweden
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- Abreu, Ilka (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology,Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden
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- Bygdell, Joakim (author)
- Umeå universitet,Kemiska institutionen
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- Hertzberg, M. (author)
- SweTree Technologies, Umeå, Sweden
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- Hvidsten, Torgeir R., 1975- (author)
- Umeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC)
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- Moritz, Thomas (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology,Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden
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- Wingsle, Gunnar (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för skoglig genetik och växtfysiologi,Department of Forest Genetics and Plant Physiology,Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden
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- Trygg, Johan (author)
- Umeå universitet,Kemiska institutionen
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- Tuominen, Hannele, 1964- (author)
- Umeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC)
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- Jönsson, Leif J. (author)
- Umeå universitet,Kemiska institutionen
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(creator_code:org_t)
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- 2021-02-16
- 2021
- English.
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In: Biotechnology for Biofuels. - : Springer Science and Business Media LLC. - 1754-6834. ; 14:1
- Related links:
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https://biotechnolog...
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https://pub.epsilon.... (primary) (Raw object) (free)
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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https://res.slu.se/i...
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Abstract
Subject headings
Close
- Background Bioconversion of wood into bioproducts and biofuels is hindered by the recalcitrance of woody raw material to bioprocesses such as enzymatic saccharification. Targeted modification of the chemical composition of the feedstock can improve saccharification but this gain is often abrogated by concomitant reduction in tree growth. Results In this study, we report on transgenic hybrid aspen (Populus tremula x tremuloides) lines that showed potential to increase biomass production both in the greenhouse and after 5 years of growth in the field. The transgenic lines carried an overexpression construct for Populus tremula x tremuloides vesicle-associated membrane protein (VAMP)-associated protein PttVAP27-17 that was selected from a gene-mining program for novel regulators of wood formation. Analytical-scale enzymatic saccharification without any pretreatment revealed for all greenhouse-grown transgenic lines, compared to the wild type, a 20-44% increase in the glucose yield per dry weight after enzymatic saccharification, even though it was statistically significant only for one line. The glucose yield after enzymatic saccharification with a prior hydrothermal pretreatment step with sulfuric acid was not increased in the greenhouse-grown transgenic trees on a dry-weight basis, but increased by 26-50% when calculated on a whole biomass basis in comparison to the wild-type control. Tendencies to increased glucose yields by up to 24% were present on a whole tree biomass basis after acidic pretreatment and enzymatic saccharification also in the transgenic trees grown for 5 years on the field when compared to the wild-type control. Conclusions The results demonstrate the usefulness of gene-mining programs to identify novel genes with the potential to improve biofuel production in tree biotechnology programs. Furthermore, multi-omic analyses, including transcriptomic, proteomic and metabolomic analyses, performed here provide a toolbox for future studies on the function of VAP27 proteins in plants.
Subject headings
- LANTBRUKSVETENSKAPER -- Bioteknologi med applikationer på växter och djur -- Växtbioteknologi (hsv//swe)
- AGRICULTURAL SCIENCES -- Agricultural Biotechnology -- Plant Biotechnology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Bioenergi (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Bioenergy (hsv//eng)
- LANTBRUKSVETENSKAPER -- Lantbruksvetenskap, skogsbruk och fiske -- Trävetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Agriculture, Forestry and Fisheries -- Wood Science (hsv//eng)
Keyword
- Populus
- Vesicle-associated membrane protein
- VAMP
- VAMP-associated
- protein
- VAP27
- Growth
- Bioprocessing
- Transcriptomics
- Proteomics
- Metabolomics
- Biotechnology & Applied Microbiology
- Energy & Fuels
- Bioprocessing
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
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Gandla, Madhavi ...
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Mähler, Niklas
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Escamez, Sacha, ...
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Skotare, Tomas
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Obudulu, Ogonna, ...
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Moller, L.
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show more...
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Abreu, Ilka
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Bygdell, Joakim
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Hertzberg, M.
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Hvidsten, Torgei ...
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Moritz, Thomas
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Wingsle, Gunnar
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Trygg, Johan
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Tuominen, Hannel ...
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Jönsson, Leif J.
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show less...
- About the subject
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- AGRICULTURAL SCIENCES
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AGRICULTURAL SCI ...
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and Agricultural Bio ...
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and Plant Biotechnol ...
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- ENGINEERING AND TECHNOLOGY
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ENGINEERING AND ...
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and Industrial Biote ...
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and Bioenergy
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- AGRICULTURAL SCIENCES
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AGRICULTURAL SCI ...
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and Agriculture Fore ...
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and Wood Science
- Articles in the publication
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Biotechnology fo ...
- By the university
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University of Gothenburg
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Swedish University of Agricultural Sciences
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Umeå University