Sökning: onr:"swepub:oai:lup.lub.lu.se:25e86bb3-1109-47ae-9d48-a87f56c20a5f" > Carbohydrate-bindin...
Fältnamn | Indikatorer | Metadata |
---|---|---|
000 | 04252naa a2200433 4500 | |
001 | oai:lup.lub.lu.se:25e86bb3-1109-47ae-9d48-a87f56c20a5f | |
003 | SwePub | |
008 | 181114s2000 | |||||||||||000 ||eng| | |
024 | 7 | a https://lup.lub.lu.se/record/25e86bb3-1109-47ae-9d48-a87f56c20a5f2 URI |
024 | 7 | a https://doi.org/10.1042/0264-6021:34500532 DOI |
040 | a (SwePub)lu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a art2 swepub-publicationtype |
072 | 7 | a ref2 swepub-contenttype |
100 | 1 | a Abou Hachem, Maheru Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)biot-mah |
245 | 1 0 | a Carbohydrate-binding modules from a thermostable Rhodothermus marinus xylanase : Cloning, expression and binding studies |
264 | 1 | b Portland Press Ltd.c 2000 |
300 | a 8 s. | |
520 | a The two N-terminally repeated carbohydrate-binding modules (CBM4-1 and CBM4-2) encoded by xyn10A from Rhodothermus marinus were produced in Escherichia coli and purified by affinity chromatography. Binding assays to insoluble polysaccharides showed binding to insoluble xylan and to phosphoric-acid-swollen cellulose but not to Avicel or crystalline cellulose. Binding to insoluble substrates was significantly enhanced by the presence of Na+ and Ca2+ ions. The binding affinities for soluble polysaccharides were tested by affinity electrophoresis; strong binding occurred with different xylans and β-glucan. CBM4-2 displayed a somewhat higher binding affinity than CBM4-1 for both soluble and insoluble substrates but both had similar specificities. Binding to short oligosaccharides was measured by NMR; both modules bound with similar affinities. The binding of the modules was shown to be dominated by enthalpic forces. The binding modules did not contribute with any significant synergistic effects on xylan hydrolysis when incubated with a Xyn10A catalytic module. This is the first report of family 4 CBMs with affinity for both insoluble xylan and amorphous cellulose. | |
650 | 7 | a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng |
653 | a CBD | |
653 | a Hemicellulose binding | |
653 | a Modular proteins | |
653 | a Xyn10A | |
700 | 1 | a Nordberg Karlsson, Evau Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)biot-eno |
700 | 1 | a Bartonek-Roxå, Evau Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)med-ebx |
700 | 1 | a Raghothama, Srinivasraou University of Sheffield4 aut |
700 | 1 | a Simpson, Peter J.u University of Sheffield4 aut |
700 | 1 | a Gilbert, Harry J.u University of Newcastle upon Tyne4 aut |
700 | 1 | a Williamson, Michael P.u University of Sheffield4 aut |
700 | 1 | a Holst, Olleu Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH4 aut0 (Swepub:lu)biot-oho |
710 | 2 | a Bioteknikb Centrum för tillämpade biovetenskaper4 org |
773 | 0 | t Biochemical Journald : Portland Press Ltd.g 345:1, s. 53-60q 345:1<53-60x 0264-6021 |
856 | 4 | u http://dx.doi.org/10.1042/0264-6021:3450053y FULLTEXT |
856 | 4 | u https://europepmc.org/articles/pmc1220729?pdf=render |
856 | 4 8 | u https://lup.lub.lu.se/record/25e86bb3-1109-47ae-9d48-a87f56c20a5f |
856 | 4 8 | u https://doi.org/10.1042/0264-6021:3450053 |
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