onr:"swepub:oai:lup.lub.lu.se:5abc76a7-e371-4202-945e-9393908ba806"
Search: onr:"swepub:oai:lup.lub.lu.se:5abc76a7-e371-4202-945e-9393908ba806" > A simple and fast l...
- 1 of 1
- Previous record
- Next record
- To hitlist
A simple and fast liquid-liquid extraction method for the determination of 2,2 ',4,4 ',5,5 '-hexachlorobiphenyl (CB-153) and 1,1-dichloro-2,2-bis(p-chlorophenyl)-ethylene (p,p '-DDE) from human serum for epidemiological studies on type 2 diabetes
- Rantakokko, Panu (author)
- Kiviranta, Hannu (author)
-
- Rylander, Lars (author)
- Lund University,Lunds universitet,Avdelningen för arbets- och miljömedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Occupational and Environmental Medicine, Lund University,Department of Laboratory Medicine,Faculty of Medicine
- show more...
- Vartiainen, Terttu (author)
- show less...
- (creator_code:org_t)
- Elsevier BV, 2009
- 2009
- English.
- In: Journal of Chromatography A. - : Elsevier BV. - 0021-9673. ; 1216:6, s. 897-901
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- A simple and fast method is presented to be used for example in studies on the relationship between serum levels of persistent organic pollutants and type 2 diabetes mellitus. Method is based on liquid-liquid extraction and gas chromatography coupled with high.-resolution mass spectrometry. In the sample pretreatment special attention was paid to minimize the number of sample manipulation steps and the amounts of organic solvents needed. Compounds analyzed were 2,2',4,4',5,5'-hexachlorobiphenyl (PCB-153) and 1,1-dichloro-2,2-bis (p-chlorophenyl)-ethylene (p,p'-DDE), the major metabolite of DDT. The method included extraction and cleanup of 0.2 rill of serum in a single test tube and subsequent analysis of the extract from 0.2 ml final volume. Validation was conducted to explore the performance of the method. The limits of detection for p,p'-DDE and PCB-153 based on the standard deviation of the blank samples were 4.3 and 3.1 pg/ml, respectively. Repeatability was less than 2.5% at three concentration levels tested and recovery from Certified Reference Material SRM 1589a was 84% for p,p'-DDE and 87% for PCB-153 of the certified values, respectively. Serum samples from the AMAP intercalibration round 2008-2 were also analyzed, and results were 101-116% of the assigned values. The presented method was used for an epidemiological study with more than 700 serum samples from a type 2 diabetes cohort from Sweden. (C) 2008 Elsevier B.V. All rights reserved.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Hälsovetenskap -- Arbetsmedicin och miljömedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Health Sciences -- Occupational Health and Environmental Health (hsv//eng)
Keyword
- PCB-153
- p
- p '-DDE
- organic pollutants (POPs)
- Persistent
- Type 2 diabetes
- Liquid-liquid extraction
- Human serum
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
- Journal of Chromatography A (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Rantakokko, Panu
- Kiviranta, Hannu
- Rylander, Lars
- Rignell-Hydbom, ...
- Vartiainen, Tert ...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Health Sciences
- and Occupational Hea ...
- Articles in the publication
- Journal of Chrom ...
- By the university
- Lund University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
