Probing Physical Properties of a DNA- Protein Complex Using Nanofluidic Channels

• A method to investigate physical properties of a DNA-protein complex in solution is demonstrated. By using tapered nanochannels and lipid passivation the persistence length of a RecA filament formed on double-stranded DNA is determined to 1.15 μm, in agreement with the literature, without attaching protein or DNA to any handles or surfaces.

Ämnesord

NATURVETENSKAP  -- Biologi -- Biofysik (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biophysics (hsv//eng)

Nyckelord

DNA

fluorescence

lipid passivation

nanofluidics

proteins

Publikations- och innehållstyp

ref (ämneskategori)

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