| 1. |
- Addario, Barbara, et al.
(författare)
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The domain structure of Entamoeba α-actinin2
- 2010
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Ingår i: Cellular & Molecular Biology Letters (Druk). - : Springer. - 1425-8153 .- 1689-1392. ; 15:4, s. 665-78
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Tidskriftsartikel (refereegranskat)abstract
- Entamoeba histolytica, a major agent of human amoebiasis, expresses two distinct forms of α-actinin, a ubiquitous actin-binding protein that is present in most eukaryotic organisms. In contrast to all metazoan α-actinins, in both isoforms the intervening rod domain that connects the N-terminal actin-binding domain with the C-terminal EF-hands is much shorter. It is suggested that these α-actinins may be involved in amoeboid motility and phagocytosis, so we cloned and characterised each domain of one of these α-actinins to better understand their functional role. The results clearly showed that the domains have properties very similar to those of conventional α-actinins.
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| 2. |
- Akhtar, M., et al.
(författare)
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Evaluation of the safety and immunogenicity of the oral inactivated multivalent enterotoxigenic Escherichia coli vaccine ETVAX in Bangladeshi adults in a double-blind, randomized, placebo-controlled Phase I trial using electrochemiluminescence and ELISA assays for immunogenicity analyses
- 2019
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Ingår i: Vaccine. - : Elsevier BV. - 0264-410X. ; 37:37, s. 5645-5656
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Tidskriftsartikel (refereegranskat)abstract
- The safety and immunogenicity of the second generation oral enterotoxigenic Escherichia coli (ETEC) vaccine ETVAX, consisting of inactivated recombinant E. coli strains over-expressing the colonization factors (CFs) CFA/I, CS3, CS5 and CS6 and the heat labile toxoid LCTBA, were evaluated in Bangladeshi volunteers. To enable analysis of antibody responses against multiple vaccine antigens for subsequent use in small sample volumes from children, a sensitive electrochemiluminescence (ECL) assay for analysis of intestine-derived antibody-secreting cell responses using the antibodies in lymphocyte secretions (ALS) assay was established using Meso Scale Discovery technology. Three groups of Bangladeshi adults (n = 15 per group) received two oral doses of ETVAX with or without double mutant LT (dmLT) adjuvant or placebo in the initial part of a randomized, double-blind, placebo-controlled, age-descending, dose-escalation trial. CF- and LTB-specific ALS and plasma IgA responses were analyzed by ECL and/or ELISA. ETVAX was safe and well tolerated in the adults. Magnitudes of IgA ALS responses determined by ECL and ELISA correlated well (r = 0.85 to 0.98 for the five primary antigens, P < 0.001) and ECL was selected as the ALS readout method. ALS IgA responses against each of the primary antigens were detected in 87-100% of vaccinees after the first and in 100% after the second vaccine dose. Plasma IgA responses against different CFs and LTB were observed in 62-93% and 100% of vaccinees, respectively. No statistically significant adjuvant effect of dmLT on antibody responses to any antigen was detected, but the overall anti-genic breadth of the plasma IgA response tended to favor the adjuvanted vaccine when responses to 4 or more or 5 vaccine antigens were considered. Responses in placebo recipients were infrequent and mainly detected against single antigens. The promising results in adults supported testing ETVAX in descending age groups of children.
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| 3. |
- Akkoyun, S., et al.
(författare)
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AGATA - Advanced GAmma Tracking Array
- 2012
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Ingår i: Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment. - : Elsevier BV. - 0168-9002 .- 0167-5087 .- 1872-9576. ; 668, s. 26-58
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Tidskriftsartikel (refereegranskat)abstract
- The Advanced GAmma Tracking Array (AGATA) is a European project to develop and operate the next generation γ-ray spectrometer. AGATA is based on the technique of γ-ray energy tracking in electrically segmented high-purity germanium crystals. This technique requires the accurate determination of the energy, time and position of every interaction as a γ ray deposits its energy within the detector volume. Reconstruction of the full interaction path results in a detector with very high efficiency and excellent spectral response. The realisation of γ-ray tracking and AGATA is a result of many technical advances. These include the development of encapsulated highly segmented germanium detectors assembled in a triple cluster detector cryostat, an electronics system with fast digital sampling and a data acquisition system to process the data at a high rate. The full characterisation of the crystals was measured and compared with detector- response simulations. This enabled pulse-shape analysis algorithms, to extract energy, time and position, to be employed. In addition, tracking algorithms for event reconstruction were developed. The first phase of AGATA is now complete and operational in its first physics campaign. In the future AGATA will be moved between laboratories in Europe and operated in a series of campaigns to take advantage of the different beams and facilities available to maximise its science output. The paper reviews all the achievements made in the AGATA project including all the necessary infrastructure to operate and support the spectrometer. © 2011 Elsevier B.V. All rights reserved.
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| 6. |
- Genheden, Samuel
(författare)
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On the estimation of ligand binding affinities
- 2012
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- A method to accurately estimate the binding affinity of a small molecule to a receptor would be indispensable in numerous fields. For instance, most drugs exert their action by binding to a macromolecule target. Thus, a lot of time and resources could be saved in drug design by predicting affinities by computer programs. In a series of 15 papers, we have tested, compared, and improved the most popular methods to estimate binding affinities. We have used for instance molecular mechanics with generalized Born and surface area solvation (MM/GBSA), linear interaction energy (LIE), and alchemical perturbation methods. Some of the topics covered are: * How the precision of MM/GBSA estimates are affected by the simulation protocol * If semiempirical quantum-mechanical methods can improve affinity estimates * A comparison of different polar solvation methods in MM/GBSA * If non-polar solvation methods can model different degrees of active-site hydration * How to obtain normal-mode entropies accurately and efficiently * What method is more efficient: LIE or MM/GBSA * A comparison of several end-point continuum-solvation methods * What charge model to use in simulations of host–guest complexes * The performance of end-point methods in a binding-affinity blind test * How we can make alchemical methods more useful for drug design * If a single-reference state can be used to simulate several ligands * What properties calculated from molecular dynamics simulations do converge Together, these studies clearly show what methods to use and what methods to avoid. We conclude that approximate methods are not very accurate and the results are highly system dependent. On the other hand, using alchemical methods, affinity differences between similar ligands can be accurately estimated both quickly and with a high precision.
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| 7. |
- Huang, Fei, et al.
(författare)
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Modeling 3D time-lapse seismic response induced by CO2 by integrating borehole and 3D seismic data - A case study at the Ketzin pilot site, Germany
- 2015
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Ingår i: International Journal of Greenhouse Gas Control. - : Elsevier BV. - 1750-5836 .- 1878-0148. ; 36, s. 66-77
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Tidskriftsartikel (refereegranskat)abstract
- In order to simulate the 3D time-lapse seismic response from real data in a consistent manner detailed 3D property models at the Ketzin pilot site were constructed by robustly integrating borehole and 3D reflection seismic data. The spatial CO2 distribution and the detailed CO2 density in the reservoir were derived from dynamic flow simulations that had been history-matched to the site monitoring data. Changes in velocity and density after CO2 injection were estimated utilizing the CO2 saturation distributions at two repeat times and fluid substitution models. 4D seismic data were generated by convolving the property models at different times with an extracted wavelet. Time-lapse analysis was performed to qualitatively and quantitatively investigate the changes in reflection amplitude after CO2 injection. Comparison between the synthetic and real data at the corresponding time indicates that the 3D property models were built successfully and model the 3D time-lapse seismic response induced by CO2 injection. A synthetic experiment with two different source wavelets was implemented to investigate the impact of source non-repeatability on the seismic amplitude anomaly. Analysis shows that the same or similar sources should be used in time-lapse seismic monitoring to minimize the impact of source non-repeatability on the monitoring.
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| 8. |
- Kuhlin, Fanny, 1994-, et al.
(författare)
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Long-term impact of the coach-athlete relationship on development, health, and wellbeing : stories from a figure skater
- 2020
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Ingår i: Sports Coaching Review. - : Routledge. - 2164-0629 .- 2164-0637. ; 9:2, s. 208-230
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Tidskriftsartikel (refereegranskat)abstract
- Coaches have been shown to detriment athletes’ health, well-being and development. Knowledge of this long-term effect and what it means for athletes to live with such stories is under-explored. Using self-narrative, we examine the long-lasting impact of the coach-athlete relationship in the stories of a former figure skater, Fanny. Guided by Arthur Frank’s dialogical analysis, we present creative non-fictional stories to show how Fanny made sense of her figure skating experiences, which were framed by a sport investment narrative and a career-wrecking injury that terminated her dream of becoming a professional figure skater. We suggest that if handled as an act of self-care, storytelling can re-configure the dominant coach-athlete relationship and sport investment narrative and help athletes to understand and reconstruct their stories. Finally, we reflect upon the impact of Fanny’s story on her advisers and consider the pedagogical implications of such narrative work in sport coaching and sport education.
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| 9. |
- Kuhlin, Joel
(författare)
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A Beautiful Failure : The Event of Death and Rhetorical Disorder in the Gospel according to Mark
- 2024
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Is there beauty in rhetorical failure? This study is an exploration of disorder and death in the Gospel according to Mark (Mk). With a surviving fragment from the second-century theologian Papias of Hierapolis, the early reception of Mk locates insights into the composition of Jesus’s death, especially through the concepts of ataxia and rhetorical failure. Papias launches an early and influential defense of its unsystematic presentation (Hist. eccl. 3.39.1–17), understood to be a kind of failure (hamartêma). By exploring this Papian doxa and heuristically accepting “disorderly failure” as a characteristic of Mk in general and 15:6–39 in particular, the present study seeks to determine a sense of Jesus’s death, directly linking an articulation of the shameful event of Jesus’s execution on a suspension-pole to the methodological and theoretical appreciation of disorder in a rhetorical composition.The study first investigates the structural (in)coherency in Mk. Going as far back as the school of Formgeschichte in the 1920s, a widespread scholarly opinion finds the content of the passion narrative (Mk 14:1–16:8) to resist its otherwise largely incoherent narrative structure. Thus, I ask: What does a progymnastic analysis reveal about the episodic order, style, and thought of Jesus’s death in Mk 15:6–39? With a methodological perspective informed by Aelius Theon’s Progymnasmata (first century CE) and the concepts of order (τάξις: taxis), style (λέξις: lexis), and thought (διανοία: dianoia), the passion narrative unfolds with a series of basic narrational episodes of “short stories” (διηγήματα: diêgêmata) and “anecdotes” (χρεῖαι: chreiai). In relation to these episodes’ inner organization and the organization of the narrational elements of person (πρόσωπον: prosôpon) and happening (πρᾶγμα; πρᾶξις: pragma; praxis), Jesus’s death scene is a particularly troubled section. Yet its rhetorical idiosyncrasies are not limited to a presentation of “persons-in-action.” The passage is filled with aspects of humiliation, obscurity, secrecy, and a paradoxical rendition of the protagonist’s final hours, while also investing its story with apocalyptic imagery and a declaration of Jesus as “a son of God,” post-mortem. The Papian doxa therefore adequately summarize the acme of this ancient narrative.With the Papian belief in truth subsisting in ataxia, failure does not need to be an end of sense and beauty. I therefore also ask: what sense and rhetorical meaning of Jesus’s death result from a progymnastic-like composition of disorder in Mk 15:6–39? In light of Walter Benjamin’s interpretation of Franz Kafka, the exploration of Joseph K.’s execution in The Trial (Der Prozeß) underlines similarities to the symbolically loaded, yet obscure, death-event in Mk 15:6–39. An analysis of K.’s demise points toward an effectual rhetoric, dedicated to expressing a de-humanizing process of dying and its final actualization through a metamorphosis into an animal-like state. The prose of K.’s end rearticulates central features found in Jesus’s death, not least a “becoming-animal” of social death. Further, while Theon’s Progymnasmata and the rhetoric of the Greco-Roman antiquity helped identify disorder and failure in Mk, the same perspective nonetheless struggles with the task of attributing the ataxia in Mk 15:6–39 with meaning. Turning to the contemporary French philosopher Gilles Deleuze and the concept of the “event” in his Logic of Sense in particular, disorderly failure is allowed to speak on its own terms. Building especially upon the results from the methodological and progymnastic analysis, Logic of Sense and the concept of event underline signs of novelty of thought and literary innovation in the ancient rhetoric of Jesus’s death. Logic of Sense assists in an evaluation of the failure of disorder in Mk 15:6–39, drawing attention to its ability to express the sense of an event. As event, I argue, the ataxia of Jesus’s death finds theoretical resources for constructing a “rhetoric of failure,” and thereby affirms the literary potential of the Papian doxa and adamant belief in the truth and perhaps even a divine beauty of failure. Lastly, imagining the sense and “sensation” of Jesus’s death, Deleuze’s exegesis of painter Francis Bacon and the motif of “crucifixion” in his art becomes crucial. Extending the line of argument found in Logic of Sense, Bacon’s vision of suspended and decaying bodies, the grotesque and creative event at play in Mk 15:6–39 gains even more determination.
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| 10. |
- Ma, Xiaoliang
(författare)
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Towards Intelligent Fleet Management : Local Optimal Speeds for Fuel and Emissions
- 2013
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Ingår i: Proceedings of the 16th IEEE International Conference on Intelligent Transportation Systems (IEEE ITSC 2013). - Den Haag, Netherland : IEEE conference proceedings.
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Konferensbidrag (refereegranskat)abstract
- In order to fulfill the policy requirements on increased transport energy efficiency and reduced emission impacts, smart control and management of vehicles and fleets have become important for the evolution of green intelligent transportation systems (ITS). The emergence of new information and communication technologies (ICT) and their applications, especially vehicle-to-vehicle and vehicle-to-infrastructure (V2I) communication, serves as an effective means for continuous management of real traffic fleet by providing vehicle driving support and guidance, and therefore affecting driver behavior. This study presents a recent Swedish R&D project for developing a dynamic fleet management system that incorporates real-time traffic information, eco-driving guidance and automated vehicle control in real-time heavy vehicle platooning. In addition to a general illustration of the main objectives of the project, the paper presents a methodological approach to developed local fleet control strategies so that the fuel and emissions of the managed vehicle fleet can be reduced. Speed trajectories minimizing predefined objectives are derived by applying a discrete dynamic programming method, and an instantaneous emission estimator is used for predicting fuel and emissions. Numerical examples show that the method is promising for real-time fleet management applications with support of V2I communication while the computational efficiency of the method needs to be enhanced. The adaptive speed control approach is implemented in a microscopic traffic simulation environment for further evaluation.
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