| 341. |
- Pejler, Gunnar, et al.
(författare)
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Serglycin- the master of the mast cell
- 2012
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Ingår i: Methods in Molecular Biology. - Totowa, NJ : Humana Press. - 1064-3745 .- 1940-6029. ; 836, s. 201-217
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Tidskriftsartikel (refereegranskat)
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| 342. |
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| 343. |
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| 344. |
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| 345. |
- Persson, Andrea, et al.
(författare)
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Production and HPLC-Based Disaccharide Analysis of Xyloside-Primed Glycosaminoglycans
- 2022
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Ingår i: Glycosaminoglycans. Methods in Molecular Biology, vol 2303. Balagurunathan K., Nakato H., Desai U., Saijoh Y. (eds). - New York, NY : Springer. - 1064-3745 .- 1940-6029. - 9781071613986 ; , s. 173-182
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Although glycosaminoglycans (GAGs) are known to be involved in a variety of physiological and pathological processes, knowledge about their expression by cells or tissues, the GAGome, is limited. Xylosides can be used to induce the formation of GAGs without the presence of a proteoglycan core protein. The administration of xylosides to living cells tends to result in a considerable amplification in GAG production, and the xylosides can, therefore, be used as analytical tools to study the GAG produced by a certain cell type. One of the most common ways to analyze the GAGs structurally is by disaccharide analysis, which involves depolymerization of the GAGs into disaccharides, fluorescent labeling of the disaccharides with 2-aminoacridone, and quantification using high-pressure liquid chromatography (HPLC). Here, we describe the procedure of producing xyloside-primed GAGs and how to study them structurally by disaccharide analysis. © 2022, Springer Science+Business Media, LLC, part of Springer Nature.
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| 346. |
- Persson, Helena, et al.
(författare)
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Antibody validation by immunoprecipitation followed by mass spectrometry analysis
- 2017
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Ingår i: Methods in Molecular Biology. - New York, NY : Humana Press Inc.. ; 1575, s. 175-187
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Konferensbidrag (refereegranskat)abstract
- We describe a mass spectrometry-based approach for validation of antibody specificity. This method allows validation of antibodies or antibody fragments, against their endogenous targets. It can assess if the antibody is able to bind to its native antigen in cell lysates among thousands of other proteins, DNA, RNA, and other cellular components. In addition, it identifies other proteins the antibody is able to immunoprecipitate allowing for the assessment of antibody specificity and selectivity. This method is easily scalable, adaptable to different cell lines and conditions and has been shown to be reproducible between multiple laboratories.
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| 347. |
- Pettersson, Mats, et al.
(författare)
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Capacitating epistasis--detection and role in the genetic architecture of complex traits.
- 2015
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Ingår i: Methods in Molecular Biology. - New York, NY : Springer New York. - 1064-3745 .- 1940-6029. ; 1253:1253, s. 185-196
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Tidskriftsartikel (refereegranskat)abstract
- Here, we discuss the potential role of capacitating epistasis in the genetic architecture of complex traits. Two alternative methods for identifying such gene-gene interactions in genetic association studies-mapping of variance controlling loci and the variance plane ratio (VPR) method-are introduced. An overview of the theoretical foundation of the methods is presented together with a discussion on their implementation and available software for performing these analyses. We conclude by highlighting a few examples of capacitating epistasis described in the literature and its potential impacts on the genetics of complex traits.
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| 348. |
- Pfeiffer, Indriati, 1974, et al.
(författare)
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Formation of pit-spanning phospholipid bilayers on nanostructured silicon dioxide surfaces for studying biological membrane events
- 2013
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Ingår i: Methods in Molecular Biology. - Totowa, NJ : Humana Press. - 1940-6029 .- 1064-3745. - 9781627033350 ; 991, s. 113-125
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Zwitterionic phospholipid vesicles are known to adsorb and ultimately rupture on flat silicon dioxide (SiO 2 ) surfaces to form supported lipid bilayers. Surface topography, however, alters the kinetics and mechanistic details of vesicles adsorption, which under certain conditions may be exploited to form a suspended bilayer. Here we describe the use of nanostructured SiO 2 surfaces prepared by the colloidal lithography technique to scrutinize the formation of suspended 1-palmitoyl-2-oleoyl-sn-glycero-3- phosphocholine (POPC) lipid bilayers from a solution of small unilamellar lipid vesicles (SUV s ). Atomic force microscopy (AFM) and quartz crystal microbalance with dissipation monitoring (QCM-D) were employed to characterize nanostructure fabrication and lipid bilayer assembly on the surface. © 2013 Springer Science+Business Media New York.
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| 349. |
- Pielberg, Gerli, et al.
(författare)
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Gene copy number detection in animal studies
- 2007
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Ingår i: Methods in Molecular Biology. - New Jersey : Humana Press. - 1064-3745 .- 1940-6029. ; 373, s. 147-156
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Tidskriftsartikel (refereegranskat)abstract
- Sensitive methods for the quantification of DNA fragments can be used to study an individual's genetic constitution for duplicated regions of the genome or to determine the relative proportion of different DNA fragments in heterogeneous samples such as pooled DNA from different individuals or in samples in which a fraction of the chromosomes carry a mutation. Here, we describe how we are using Pyrosequencing for this purpose. In Subheading 3., we describe a sensitive method that can be used to quantify the relative proportion of X- and Y-carrying sperm after sperm sorting in cattle. We also discuss our method for determining the copy numbers at a duplicated locus. This method has been applied to study genetic variation at the KIT locus in pigs, which have a major effect on coat color variation in this species.
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| 350. |
- Pigot, Harry, et al.
(författare)
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Ex Vivo Working Porcine Heart Model
- 2024. - 2
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Ingår i: Experimental Models of Cardiovascular Diseases : Methods and Protocols - Methods and Protocols. - 1064-3745 .- 1940-6029. - 9781071638460 - 9781071638484 - 9781071638453 ; 2803, s. 87-107
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Ex vivo working porcine heart models allow for the study of a heart’s function and physiology outside the living organism. These models are particularly useful due to the anatomical and physiological similarities between porcine and human hearts, providing an experimental platform to investigate cardiac disease or assess donor heart viability for transplantation. This chapter presents an in-depth discussion of the model’s components, including the perfusate, preload, and afterload. We explore the challenges of emulating cardiac afterload and present a historical perspective on afterload modeling, discussing various methodologies and their respective limitations. An actively controlled afterload device is introduced to enhance the model’s ability to rapidly adjust pressure in the large arteries, thereby providing a more accurate and dynamic experimental model. Finally, we provide a comprehensive experimental protocol for the ex vivo working porcine heart model.
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