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Search: onr:"swepub:oai:DiVA.org:uu-94699" > Screening of an ann...

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  • Rickardson, LindaUppsala universitet,Klinisk farmakologi,Cancer Pharmacology and Informatics (author)

Screening of an annotated compound library for drug activity in a resistant myeloma cell line

  • Article/chapterEnglish2006

Publisher, publication year, extent ...

  • 2006-03-10
  • Springer Science and Business Media LLC,2006
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:uu-94699
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-94699URI
  • https://doi.org/10.1007/s00280-006-0216-7DOI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Purpose: Resistance to anticancer drugs is a major problem in chemotherapy. In order to identify drugs with selective cytotoxic activity in drug-resistant cancer cells, the annotated compound library LOPAC(1280), containing compounds from 56 pharmacological classes, was screened in the myeloma cell line RPMI 8226 and its doxorubicin-resistant subline 8226/Dox40. Methods: Cell survival was measured by the Fluorometric Microculture Cytotoxicity Assay. Results: Selective cytotoxic activity in 8226/Dox40 was obtained for 33 compounds, with the most pronounced difference observed for the glucocorticoids. A microarray analysis of the cells showed a difference in mRNA-expression for the glucocorticoid receptor suggesting potential mechanisms for the difference in glucocorticoid sensitivity. In the presence of the glucocorticoid-receptor antagonist RU486, the sensitivity to the glucocorticoids was reduced and a similar effect level in RPMI 8226 and 8226/Dox40 was achieved. Conclusion: In conclusion, screening of mechanistically annotated compounds on drug-resistant cancer cells can identify compounds with selective activity and provide a basis for the development of novel treatments of drug-resistant malignancies.

Subject headings and genre

  • anticancer drug resistance
  • drug screening
  • annotated compound library
  • glucocorticoids
  • gene expression
  • MEDICINE
  • MEDICIN

Added entries (persons, corporate bodies, meetings, titles ...)

  • Fryknäs, MårtenUppsala universitet,Institutionen för genetik och patologi,Cancer Pharmacology and Informatics(Swepub:uu)mafry516 (author)
  • Haglund, CarolineUppsala universitet,Klinisk farmakologi,Cancer Pharmacology and Informatics(Swepub:uu)cahag030 (author)
  • Lövborg, HenrikUppsala universitet,Klinisk farmakologi,Cancer Pharmacology and Informatics (author)
  • Nygren, PeterUppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi,Cancer Pharmacology and Informatics(Swepub:uu)peterng (author)
  • Gustafsson, MatsUppsala universitet,Institutionen för genetik och patologi,Signalbehandling,Cancer Pharmacology and Informatics(Swepub:uu)matsgust (author)
  • Isaksson, AndersUppsala universitet,Institutionen för genetik och patologi,Cancer Pharmacology and Informatics(Swepub:uu)andeisak (author)
  • Larsson, RolfUppsala universitet,Klinisk farmakologi,Cancer Pharmacology and Informatics (author)
  • Uppsala universitetKlinisk farmakologi (creator_code:org_t)

Related titles

  • In:Cancer Chemotherapy and Pharmacology: Springer Science and Business Media LLC58:6, s. 749-7580344-57041432-0843

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