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Screening of an ann...
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Rickardson, LindaUppsala universitet,Klinisk farmakologi,Cancer Pharmacology and Informatics
(författare)
Screening of an annotated compound library for drug activity in a resistant myeloma cell line
- Artikel/kapitelEngelska2006
Förlag, utgivningsår, omfång ...
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2006-03-10
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Springer Science and Business Media LLC,2006
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:uu-94699
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-94699URI
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https://doi.org/10.1007/s00280-006-0216-7DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Purpose: Resistance to anticancer drugs is a major problem in chemotherapy. In order to identify drugs with selective cytotoxic activity in drug-resistant cancer cells, the annotated compound library LOPAC(1280), containing compounds from 56 pharmacological classes, was screened in the myeloma cell line RPMI 8226 and its doxorubicin-resistant subline 8226/Dox40. Methods: Cell survival was measured by the Fluorometric Microculture Cytotoxicity Assay. Results: Selective cytotoxic activity in 8226/Dox40 was obtained for 33 compounds, with the most pronounced difference observed for the glucocorticoids. A microarray analysis of the cells showed a difference in mRNA-expression for the glucocorticoid receptor suggesting potential mechanisms for the difference in glucocorticoid sensitivity. In the presence of the glucocorticoid-receptor antagonist RU486, the sensitivity to the glucocorticoids was reduced and a similar effect level in RPMI 8226 and 8226/Dox40 was achieved. Conclusion: In conclusion, screening of mechanistically annotated compounds on drug-resistant cancer cells can identify compounds with selective activity and provide a basis for the development of novel treatments of drug-resistant malignancies.
Ämnesord och genrebeteckningar
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anticancer drug resistance
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drug screening
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annotated compound library
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glucocorticoids
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gene expression
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MEDICINE
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MEDICIN
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Fryknäs, MårtenUppsala universitet,Institutionen för genetik och patologi,Cancer Pharmacology and Informatics(Swepub:uu)mafry516
(författare)
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Haglund, CarolineUppsala universitet,Klinisk farmakologi,Cancer Pharmacology and Informatics(Swepub:uu)cahag030
(författare)
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Lövborg, HenrikUppsala universitet,Klinisk farmakologi,Cancer Pharmacology and Informatics
(författare)
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Nygren, PeterUppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi,Cancer Pharmacology and Informatics(Swepub:uu)peterng
(författare)
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Gustafsson, MatsUppsala universitet,Institutionen för genetik och patologi,Signalbehandling,Cancer Pharmacology and Informatics(Swepub:uu)matsgust
(författare)
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Isaksson, AndersUppsala universitet,Institutionen för genetik och patologi,Cancer Pharmacology and Informatics(Swepub:uu)andeisak
(författare)
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Larsson, RolfUppsala universitet,Klinisk farmakologi,Cancer Pharmacology and Informatics
(författare)
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Uppsala universitetKlinisk farmakologi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Cancer Chemotherapy and Pharmacology: Springer Science and Business Media LLC58:6, s. 749-7580344-57041432-0843
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