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Three-dimensional c...
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Nilsang, SuthasineeLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,Lund University, Lund, Sweden; School of Environmental, Resources and Development, Asian Institute of Technology, Pathumtani, Thailand; Valaya Alongkorn Rajabhat University, Pathumtani, Thailand
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Three-dimensional culture for monoclonal antibody production by hybridoma cells immobilized in macroporous gel particles
- Artikel/kapitelEngelska2008
Förlag, utgivningsår, omfång ...
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Malden, MA :Wiley-Blackwell Publishing Inc.2008
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:hh-48845
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https://urn.kb.se/resolve?urn=urn:nbn:se:hh:diva-48845URI
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https://doi.org/10.1002/btpr.28DOI
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https://lup.lub.lu.se/record/1283665URI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:117813961URI
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Språk:engelska
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Sammanfattning på:engelska
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Funding text: The work was financially supported by Protista Biotechnology AB (Lund, Sweden), Swedish Research Council/SIDA-research link project (A.K.), the Swedish Research Council (R.H.), the EU project LSHB-CT-2006-018661 (AUTOCURE), and LSHG-CT-2005-005203 (MUGEN), Department of Biotechnology, Govt. of India. S.N. and V.N. acknowledge the financial support from Swedish Research Council/SIDAresearch link project of A.K. for visiting fellowship to Lund, Sweden.
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Cell proliferation and long-term production of monoclonal antibody IgG(2b) by M2139 hybridoma cells immobilized in macroporous gel particles (MGPs) in packed-bed reactor were studied for a period of 60 days. The MGPs were made of supermacroporous gels produced in frozen conditions from crosslinked polyacrylamide and modified with gelatin which were housed in special plastic carriers (7 x 9 mm(2)). Cells were trapped in the interior part of MGPs by attaching to the void space of the gel matrix as three-dimensional (3D) cultivation using gelatin as a substrate layer. Optimizing productivity by hybridoma cell relies on understanding regulation of antibody production. In this study, the behavior of M2139 cells in two-dimensional cultures on multiwell plate surfaces was also investigated. The effect of three different medium such as basal medium Dulbecco's modified Eagle's medium (D-MEM) containing L-glutamine or L-glutamine + 2 mM alpha-ketoglutarate or L-alanyl-glutamine (GlutaMAXtrade mark) was studied prior to its use in 3D cultivation. The kinetics of cell growth in basal medium containing L-glutamine + alpha-ketoglutarate was similar to cells grown on GlutaMAX containing medium, whereas D-MEM containing L-glutamine showed lower productivity. With the maximal viable cell density (6.85 x 10(6) cells mL(-1)) and highest specific mAb production rate (3.9 mug mL(-1) 10(-4) viable cell day(-1)), D-MEM-GlutaMAX was further selected for 3D cultivation. Cells in MGPs were able to grow and secrete antibody for 30 days in packed-bed batch reactor, before a fresh medium reservoir was replaced. After being supplied with fresh medium, cells again showed continuous growth for another 30 days with mAb production efficiency of 50%. These results demonstrate that MGPs can be used efficiently as supporting carrier for long-term monoclonal antibody production. © 2008 American Institute of Chemical Engineers.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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Nehru, VishalLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,Lund University, Lund, Sweden; Indian Institute of Technology Kanpur, Kanpur, India(Swepub:lu)biot-vnu
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Plieva, FatimaLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,Lund University, Lund, Sweden; Protista Biotechnology AB, IDEON, Lund, Sweden(Swepub:lu)biot-fpl
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Nandakumar, Kutty Selva,1965-Karolinska Institutet,Lund University,Lunds universitet,Immunologi,Forskargrupper vid Lunds universitet,Immunology,Lund University Research Groups,Lund University, Lund, Sweden(Swepub:lu)infl-nku
(författare)
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Rakshit, Sudip KumarSchool of Environmental, Resources and Development, Asian Institute of Technology, Pathumtani, Thailand
(författare)
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Holmdahl, RikardLund University,Lunds universitet,Immunologi,Forskargrupper vid Lunds universitet,Immunology,Lund University Research Groups,Lund University, Lund, Sweden(Swepub:lu)infl-rho
(författare)
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Mattiasson, BoLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,Lund University, Lund, Sweden(Swepub:lu)biot-bma
(författare)
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Kumar, AshokLund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH,Indian Institute of Technology Kanpur, Kanpur, India(Swepub:lu)as5787ku
(författare)
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BioteknikCentrum för tillämpade biovetenskaper
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Sammanhörande titlar
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Ingår i:Biotechnology progress (Print)Malden, MA : Wiley-Blackwell Publishing Inc.24:5, s. 1122-11318756-79381520-6033
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