Large-scale proteomics analysis of human ovarian cancer for biomarkers

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QC 20100525
Ovarian cancer is usually found at a late stage when the prognosis is often bad. Relative survival rates decrease with tumor stage or grade, and the 5-year survival rate for women with carcinoma is only 38%. Thus, there is a great need to find biomarkers that can be used to carry out routine screening, especially in high-risk patient groups. Here, we present a large-scale study of 64 tissue samples taken from patients at all stages and show that we can identify statistically valid markers using nonsupervised methods that distinguish between normal, benign, borderline, and malignant tissue. We have identified 217 of the significantly changing protein spots. We are expressing and raising antibodies to 35 of these. Currently, we have validated 5 of these antibodies for use in immunohistochemical analysis using tissue microarrays of healthy and diseased ovarian, as well as other, human tissues.

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Immunologi inom det medicinska området hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Immunology in the medical area hsv//eng
NATURVETENSKAP Biologi Biofysik hsv//swe
NATURAL SCIENCES Biological Sciences Biophysics hsv//eng
biomarkers
ovarian cancer
DIGE
proteomics
statistical analysis
lung-cancer
cathepsin-b
2-dimensional electrophoresis
gel-electrophoresis
protein expression
colon-cancer
cell-lines
breast
carcinoma
overexpression

Krogh, MortenLund University,Lunds universitet,Beräkningsbiologi och biologisk fysik - Genomgår omorganisation,Institutionen för astronomi och teoretisk fysik - Genomgår omorganisation,Naturvetenskapliga fakulteten,Computational Biology and Biological Physics - Undergoing reorganization,Department of Astronomy and Theoretical Physics - Undergoing reorganization,Faculty of Science(Swepub:lu)thep-mkr (author)
Al-Khalili Szigyarto, CristinaKTH,Proteomik(Swepub:kth)u1c02mh9 (author)
Uhlén, MathiasKTH,Proteomik(Swepub:kth)u1dulvmw (author)
Schedvins, Kjell (author)
Silfversward, Claes (author)
Linder, StigKarolinska Institutet (author)
Auer, GertKarolinska Institutet (author)
Alaiya, Ayodele (author)
James, PeterLund University,Lunds universitet,Institutionen för immunteknologi,Institutioner vid LTH,Lunds Tekniska Högskola,Department of Immunotechnology,Departments at LTH,Faculty of Engineering, LTH(Swepub:lu)elma-pja (author)
Beräkningsbiologi och biologisk fysik - Genomgår omorganisationInstitutionen för astronomi och teoretisk fysik - Genomgår omorganisation (creator_code:org_t)

In:Journal of Proteome Research: American Chemical Society (ACS)6:4, s. 1440-14501535-38931535-3907

By the author/editor: Bengtsson, Sofia; Krogh, Morten; Al-Khalili Szigy ...; Uhlén, Mathias; Schedvins, Kjell; Silfversward, Cl ...; show more...; Linder, Stig; Auer, Gert; Alaiya, Ayodele; James, Peter; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Immunology in th ...

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biophysics

By the university: Royal Institute of Technology; Lund University; Karolinska Institutet

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