The contribution of lipid peroxidation to membrane permeability in electropermeabilization : A molecular dynamics study

• Electroporation or electropermeabilization is a technique that enables transient increase in the cell membrane permeability by exposing cells to pulsed electric field. However, the molecular mechanisms of the long-lived cell membrane permeability, which persists on the minutes time scale after the pulse treatment, remain elusive. Experimental studies have suggested that lipid peroxidation could present a mechanism of this prolonged membrane permeabilization. In this study we make the first important step in quantifying the possible contribution of lipid peroxidation to electropermeabilization. We use free energy calculations to quantify the permeability and conductance of bilayers, containing an increasing percentage of hydroperoxide lipid derivatives, to sodium and chloride ions. We then compare our calculations to experimental measurements on electropermeabilized cells. Our results show that the permeability and conductance increase dramatically by several orders of magnitude in peroxidized bilayers. Yet this increase is not sufficient to reasonably account for the entire range of experimental measurements. Nevertheless, lipid peroxidation might be considered an important mechanism of prolonged membrane permeabilization, if exposure of cells to high voltage electric pulses leads to secondary lipid peroxidation products. Our analysis calls for experimental studies, which will determine the type and amount of lipid peroxidation products in electropermeabilized cell membranes.

Ämnesord

NATURVETENSKAP  -- Kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences (hsv//eng)

Nyckelord

Cell membrane

Electrical conductance

Electroporation

Free energy calculations

Oxidized lipids

Permeability

Cell membranes

Cells

Chlorine compounds

Electric fields

Free energy

Lipid bilayers

Lipids

Mechanical permeability

Molecular dynamics

Oxidation

Oxidative stress

Sodium compounds

Cell membrane permeability

Electropermeabilization

Free-energy calculations

Membrane permeabilization

Pulsed electric field

Cytology

chloride ion

Article

calculation

comparative study

diffusion

electric conductance

ion conductance

lipid bilayer

lipid peroxidation

membrane conductance

membrane permeability

simulation

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)