Hybrid Nanoparticle-Liposome Detection of Phospholipase Activity

• A flexible nanoparticle-based phospholipase (PL) assay is demonstrated in which the enzymatic substrate is decoupled from the nanoparticle surface. Liposomes are loaded with a polypeptide that is designed to heteroassociate with a second polypeptide immobilized on gold nanoparticies. Release of this polypeptide from the liposornes, triggered by PL, induces a folding-dependent nanoparticle bridging aggregation. The colorimetric response from this aggregation enables straightforward and continuous detection of PL in the picomolar range. The speed, specificity, and flexibility of this assay make it appropriate for a range of applications, from point of care diagnostics to high throughput pharmaceutical screening.

Ämnesord

TEKNIK OCH TEKNOLOGIER  -- Nanoteknik (hsv//swe)

ENGINEERING AND TECHNOLOGY  -- Nano-technology (hsv//eng)

Nyckelord

Phospholipase A2

gold nanoparticles

liposomes

polypeptides

helix-loop-helix

functionalized gold nanoparticles

activity

colorimetric detection

vesicles

hydrolysis

mechanism

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)