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The Role of NLRP3 i...
The Role of NLRP3 in Regulation of Antimicrobial Peptides and Estrogen Signaling in UPEC-Infected Bladder Epithelial Cells
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- Lindblad, Anna, 1987- (författare)
- Örebro universitet,Institutionen för medicinska vetenskaper
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- Wu, Rongrong, 1985- (författare)
- Örebro universitet,Institutionen för medicinska vetenskaper
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- Persson, Katarina, 1962- (författare)
- Örebro universitet,Institutionen för medicinska vetenskaper
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visa fler...
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- Demirel, Isak, 1987- (författare)
- Örebro universitet,Institutionen för medicinska vetenskaper
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visa färre...
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(creator_code:org_t)
- MDPI, 2023
- 2023
- Engelska.
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Ingår i: Cells. - : MDPI. - 2073-4409. ; 12:18
- Relaterad länk:
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https://doi.org/10.3...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- The NLRP3 inflammasome, estrogen and antimicrobial peptides have all been found to have a vital role in the protection of the bladder urothelium. However, the interdependence between these protective factors during a bladder infection is currently unknown. Our aim was to investigate the role of NLRP3 in the regulation of antimicrobial peptides and estrogen signaling in bladder epithelial cells during a UPEC infection. Human bladder epithelial cells and CRISPR/Cas9-generated NLRP3-deficient cells were stimulated with the UPEC strain CFT073 and estradiol. The gene and protein expression were evaluated with microarray, qRT-PCR, western blot and ELISA. Microarray results showed that the expression of most antimicrobial peptides was reduced in CFT073-infected NLRP3-deficient cells compared to Cas9 control cells. Conditioned medium from NLRP3-deficient cells also lost the ability to suppress CFT073 growth. Moreover, NLRP3-deficient cells had lower basal release of Beta-defensin-1, Beta-defensin-2 and RNase7. The ability of estradiol to induce an increased expression of antimicrobial peptides was also abrogated in NLRP3-deficient cells. The decreased antimicrobial peptide expression might be linked to the observed reduced expression and activity of estradiol receptor beta in NLRP3-deficient cells. This study suggests that NLRP3 may regulate the release and expression of antimicrobial peptides and affect estrogen signaling in bladder epithelial cells.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- NLRP3 inflammasome
- estradiol
- antimicrobial peptides
- uropathogenic Escherichia coli
- urinary tract infections
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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