Adherence of human oral keratinocytes and gingival fibroblasts to nano-structured titanium surfaces

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Dorkhan, MarjanMalmö högskola,Odontologiska fakulteten (OD) (författare)

Adherence of human oral keratinocytes and gingival fibroblasts to nano-structured titanium surfaces

Artikel/kapitelEngelska2014

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2014-06-21
BioMed Central,2014
electronicrdacarrier

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LIBRIS-ID:oai:DiVA.org:mau-7173
https://urn.kb.se/resolve?urn=urn:nbn:se:mau:diva-7173URI
https://doi.org/10.1186/1472-6831-14-75DOI
http://kipublications.ki.se/Default.aspx?queryparsed=id:129303697URI

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Språk:engelska
Sammanfattning på:engelska

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BACKGROUND: A key element for long-term success of dental implants is integration of the implant surface with the surrounding host tissues. Modification of titanium implant surfaces can enhance osteoblast activity but their effects on soft-tissue cells are unclear. Adherence of human keratinocytes and gingival fibroblasts to control commercially pure titanium (CpTi) and two surfaces prepared by anodic oxidation was therefore investigated. Since implant abutments are exposed to a bacteria-rich environment in vivo, the effect of oral bacteria on keratinocyte adhesion was also evaluated. METHODS: The surfaces were characterized using scanning electron microscopy (SEM). The number of adhered cells and binding strength, as well as vitality of fibroblasts and keratinocytes were evaluated using confocal scanning laser microscopy after staining with Live/Dead Baclight. To evaluate the effect of bacteria on adherence and vitality, keratinocytes were co-cultured with a four-species streptococcal consortium. RESULTS: SEM analysis showed the two anodically oxidized surfaces to be nano-structured with differing degrees of pore-density. Over 24 hours, both fibroblasts and keratinocytes adhered well to the nano-structured surfaces, although to a somewhat lesser degree than to CpTi (range 42-89% of the levels on CpTi). The strength of keratinocyte adhesion was greater than that of the fibroblasts but no differences in adhesion strength could be observed between the two nano-structured surfaces and the CpTi. The consortium of commensal streptococci markedly reduced keratinocyte adherence on all the surfaces as well as compromising membrane integrity of the adhered cells. CONCLUSION: Both the vitality and level of adherence of soft-tissue cells to the nano-structured surfaces was similar to that on CpTi. Co-culture with streptococci reduced the number of keratinocytes on all the surfaces to approximately the same level and caused cell damage, suggesting that commensal bacteria could affect adherence of soft-tissue cells to abutment surfaces in vivo.

Ämnesord och genrebeteckningar

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Odontologi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Dentistry hsv//eng
dental implant
soft-tissue
anodic oxidation
cell binding

Biuppslag (personer, institutioner, konferenser, titlar ...)

Yucel-Lindberg, TulayKarolinska Institutet (författare)
Hall, Jan (författare)
Svensäter, GunnelMalmö högskola,Odontologiska fakulteten (OD)(Swepub:mau)odgusv (författare)
Davies, JuliaMalmö högskola,Odontologiska fakulteten (OD)(Swepub:mau)odjuda (författare)
Malmö högskolaOdontologiska fakulteten (OD) (creator_code:org_t)

Sammanhörande titlar

Ingår i:BMC Oral Health: BioMed Central14:751472-68311472-6831

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Av författaren/redakt...: Dorkhan, Marjan; Yucel-Lindberg, ...; Hall, Jan; Svensäter, Gunne ...; Davies, Julia

Om ämnet

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Klinisk medicin; och Odontologi

Artiklar i publikationen: BMC Oral Health

Av lärosätet: Malmö universitet; Karolinska Institutet

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