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The interdomain reg...
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Brooks, Andrew J
(författare)
The interdomain region of dengue NS5 protein that binds to the viral helicase NS3 contains independently functional importin beta 1 and importin alpha/beta-recognized nuclear localization signals
- Artikel/kapitelEngelska2002
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Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:oru-27108
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https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-27108URI
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https://doi.org/10.1074/jbc.M204977200DOI
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https://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-6067URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Dengue virus NS5 protein is a multifunctional RNA-dependent RNA polymerase that is essential for virus replication. We have shown previously that the 37- amino acid interdomain spacer sequence (residues (369)X(2)KKX(14)KKKX(11)RKX(3)405) of Dengue2 NS5 contains a functional nuclear localization signal (NLS). In this study, beta-galactosidase fusion proteins carrying point mutations of the positively charged residues or truncations of the interdomain linker region (residues 369-389 or residues 386-405) were analyzed for nuclear import and importin binding activities to show that the N-terminal part of the linker region (residues 369-389, a/bNLS) is critical for nuclear localization and is recognized with high affinity by the conventional NLS-binding importin alpha/beta heterodimeric nuclear import receptor. We also show that the importin beta-binding site (residues 320-368, bNLS) adjacent to the a/bNLS, previously identified by yeast two-hybrid analysis, is functional as an NLS, recognized with high affinity by importin beta, and able to target beta-galactosidase to the nucleus. Intriguingly, the bNLS is highly conserved among Dengue and related flaviviruses, implying a general role for the region and importin beta in the infectious cycle.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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Johansson, MagnusJames Cook University(Swepub:sh)shmsjn02
(författare)
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John, Anna V
(författare)
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Xu, Yibin
(författare)
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Jans, David A
(författare)
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Vasudevan, Subhash G
(författare)
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James Cook University
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Journal of Biological Chemistry277:39, s. 36399-364070021-92581083-351X
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