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Streptozotocin-induced type-1-diabetes disease onset in Sprague-Dawley rats is associated with an altered intestinal microbiota composition and decreased diversity
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- Patterson, Elaine (författare)
- Alimentary Pharmabiotic Centre, Biosciences Institute, University College, Cork, Ireland; Food Biosciences Department Moorepark, Teagasc Food Research Centre, Fermoy, Ireland; Department of Microbiology, Cork, Ireland
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- Marques, Tatiana M., 1980- (författare)
- Alimentary Pharmabiotic Centre, Biosciences Institute, University College, Cork, Ireland; Food Biosciences Department Moorepark, Teagasc Food Research Centre, Fermoy, Ireland; Department of Microbiology, University College, Cork, Ireland
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- O'Sullivan, Orla (författare)
- Alimentary Pharmabiotic Centre, Biosciences Institute, University College, Cork, Ireland; Food Biosciences Department Moorepark, Teagasc Food Research Centre, Fermoy, Ireland
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- London, UK : The Microbiology Society, 2015
- 2015
- Engelska.
- Ingår i: Microbiology. - London, UK : The Microbiology Society. - 1350-0872 .- 1465-2080. ; 161:Pt 1, s. 182-93
- Tidskriftsartikel (refereegranskat)
Abstract
Ämnesord
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- There is a growing appreciation that microbiota composition can significantly affect host health and play a role in disease onset and progression. This study assessed the impact of streptozotocin (STZ)-induced type-1-diabetes (T1D) on intestinal microbiota composition and diversity in Sprague-Dawley rats, compared with healthy controls over time. T1D was induced by injection of a single dose (60 mg STZ kg(-1)) of STZ, administered via the intraperitoneal cavity. Total DNA was isolated from faecal pellets at weeks 0 (pre-STZ injection), 1, 2 and 4 and from caecal content at week 5 from both healthy and T1D groups. High-throughput 16S rRNA sequencing was employed to investigate intestinal microbiota composition. The data revealed that although intestinal microbiota composition between the groups was similar at week 0, a dramatic impact of T1D development on the microbiota was apparent post-STZ injection and for up to 5 weeks. Most notably, T1D onset was associated with a shift in the Bacteroidetes : Firmicutes ratio (P<0.05), while at the genus level, increased proportions of lactic acid producing bacteria such as Lactobacillus and Bifidobacterium were associated with the later stages of T1D progression (P<0.05). Coincidently, T1D increased caecal lactate levels (P<0.05). Microbial diversity was also reduced following T1D (P<0.05). Principle co-ordinate analyses demonstrated temporal clustering in T1D and control groups with distinct separation between groups. The results provide a comprehensive account of how T1D is associated with an altered intestinal microbiota composition and reduced microbial diversity over time.
Ämnesord
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
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- Cotter, Paul D.
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- Ross, R. Paul
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