A phylogenomic tree inferred with an inexpensive PCR-generated probe kit resolves higher-level relationships among Neptis butterflies (Nymphalidae: Limenitidinae)

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Recent advances in obtaining reduced representation libraries for next-generation sequencing permit phylogenomic analysis of species-rich, recently diverged taxa. In this study, we performed sequence capture with homemade PCR-generated probes to study diversification among closely related species in a large insect genus to examine the utility of this method. We reconstructed the phylogeny of Neptis Fabricius, a large and poorly studied nymphalid butterfly genus distributed throughout the Old World. We inferred relationships among 108 Neptis samples using 89 loci totaling up to 84 519 bp per specimen. Our taxon sample focused on Palearctic, Oriental and Australasian species, but included 8 African species and outgroups from 5 related genera. Maximum likelihood and Bayesian analyses yielded identical trees with full support for almost all nodes. We confirmed that Neptis is not monophyletic because Lasippa heliodore (Fabricius) and Phaedyma amphion (Linnaeus) are nested within the genus, and we redefine species groups for Neptis found outside of Africa. The statistical support of our results demonstrates that the probe set we employed is useful for inferring phylogenetic relationships among Neptis species and likely has great value for intrageneric phylogenetic reconstruction of Lepidoptera. Based on our results, we revise the following two taxa: Neptis heliodore comb. rev. and Neptis amphion comb. rev.

Zhang, YuanSun Yat-sen University (författare)
Lohman, David J.City University of New York,National Museum of the Philippines,City College of New York (författare)
Wahlberg, NiklasLund University,Lunds universitet,Biodiversitet,Biologiska institutionen,Naturvetenskapliga fakulteten,Biologiska museet,Systematisk biologi,Forskargrupper vid Lunds universitet,Biodiversity,Department of Biology,Faculty of Science,Biological Museum,Systematic Biology Group,Lund University Research Groups(Swepub:lu)biol-nlw (författare)
Ma, FangzhouMinistry of Environmental Protection of People's Republic of China ; Nanjing Institute of Environmental Sciences (författare)
Nylin, SörenStockholm University,Stockholms universitet,Zoologiska institutionen(Swepub:su)snylin (författare)
Janz, NiklasStockholm University,Stockholms universitet,Zoologiska institutionen(Swepub:su)janz (författare)
Yago, MasayaUniversity of Tokyo (författare)
Aduse-Poku, KwakuCity College of New York,University of Richmond (författare)
Peggie, DjunijantiMuseum Zoologicum Bogoriense (författare)
Wang, MinSouth China Agricultural University (författare)
Zhang, PengSun Yat-sen University (författare)
Wang, HoushuaiSouth China Agricultural University (författare)
South China Agricultural UniversitySun Yat-sen University (creator_code:org_t)

Av författaren/redakt...: Ma, Lijun; Zhang, Yuan; Lohman, David J.; Wahlberg, Niklas; Ma, Fangzhou; Nylin, Sören; visa fler...; Janz, Niklas; Yago, Masaya; Aduse-Poku, Kwak ...; Peggie, Djunijan ...; Wang, Min; Zhang, Peng; Wang, Houshuai; visa färre...

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