Increased expression of cSHMT, Tbx3 and utrophin in plasma of ovarian and breast cancer patients

• Plasma samples of ovarian and breast cancer patients were used to search for markers of cancer using 2-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time of flight mass spectrometry. Truncated forms of cytosolic serine hydroxymethyl transferase (cSHMT), T-box transcription factor 3 (Tbx3) and utrophin were aberrantly expressed in samples from cancer patients as compared to samples from noncancerous cases. Aberrant expression of proteins was validated by immunoblotting of plasma samples with specific antibodies to cSHMT, Tbx3 and utrophin. A cohort of 79 breast and 39 ovarian cancer patients and 31 individuals with noncancerous conditions was studied. We observed increased expression of truncated cSHMT, Tbx3 and utrophin in plasma samples obtained from patients at early stages of disease. Our data suggest that cSHMT, Tbx3 and utrophin can be used as components of multiparameter monitoring of ovarian and breast cancer (supplementary material for this article can be found on the International Journal of Cancer website at http://www.interscience.wiley.com/jpages/0020-7136/suppmat/index.html).

Nyckelord

Breast Neoplasms/*pathology

Case-Control Studies

Cytosol/chemistry

Electrophoresis; Gel; Two-Dimensional

Female

Gene Expression Profiling

Glycine Hydroxymethyltransferase/biosynthesis/*blood

Humans

Neoplasm Staging/methods

Ovarian Neoplasms/*blood

Research Support; Non-U.S. Gov't

Spectrum Analysis; Mass

T-Box Domain Proteins/biosynthesis/*blood

Tumor Markers; Biological/blood

Utrophin/biosynthesis/*blood

MEDICINE

MEDICIN

Publikations- och innehållstyp

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