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Sequencing of 15622...
Sequencing of 15622 gene-bearing BACs clarifies the gene-dense regions of the barley genome
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- Falk, Anders (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för växtbiologi,Department of Plant Biology
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(creator_code:org_t)
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- 2015-09-21
- 2015
- Engelska.
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Ingår i: Plant Journal. - : Wiley. - 0960-7412 .- 1365-313X. ; 84, s. 216-227
- Relaterad länk:
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https://onlinelibrar...
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https://res.slu.se/i...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Barley (Hordeum vulgare L.) possesses a large and highly repetitive genome of 5.1Gb that has hindered the development of a complete sequence. In 2012, the International Barley Sequencing Consortium released a resource integrating whole-genome shotgun sequences with a physical and genetic framework. However, because only 6278 bacterial artificial chromosome (BACs) in the physical map were sequenced, fine structure was limited. To gain access to the gene-containing portion of the barley genome at high resolution, we identified and sequenced 15622 BACs representing the minimal tiling path of 72052 physical-mapped gene-bearing BACs. This generated 1.7Gb of genomic sequence containing an estimated 2/3 of all Morex barley genes. Exploration of these sequenced BACs revealed that although distal ends of chromosomes contain most of the gene-enriched BACs and are characterized by high recombination rates, there are also gene-dense regions with suppressed recombination. We made use of published map-anchored sequence data from Aegilops tauschii to develop a synteny viewer between barley and the ancestor of the wheat D-genome. Except for some notable inversions, there is a high level of collinearity between the two species. The software HarvEST:Barley provides facile access to BAC sequences and their annotations, along with the barley-Ae.tauschii synteny viewer. These BAC sequences constitute a resource to improve the efficiency of marker development, map-based cloning, and comparative genomics in barley and related crops. Additional knowledge about regions of the barley genome that are gene-dense but low recombination is particularly relevant.
Ämnesord
- LANTBRUKSVETENSKAPER -- Bioteknologi med applikationer på växter och djur -- Växtbioteknologi (hsv//swe)
- AGRICULTURAL SCIENCES -- Agricultural Biotechnology -- Plant Biotechnology (hsv//eng)
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