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Sökning: onr:"swepub:oai:DiVA.org:umu-114645" > Reproducibility of ...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004432naa a2200637 4500
001oai:DiVA.org:umu-114645
003SwePub
008160125s2015 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1146452 URI
0247 a https://doi.org/10.1093/ije/dyu1912 DOI
040 a (SwePub)umu
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Martin-Ruiz, Carmen M4 aut
2451 0a Reproducibility of telomere length assessment :b an international collaborative study
264 c 2014-09-19
264 1b Oxford University Press,c 2015
338 a electronic2 rdacarrier
520 a Background: Telomere length is a putative biomarker of ageing, morbidity and mortality. Its application is hampered by lack of widely applicable reference ranges and uncertainty regarding the present limits of measurement reproducibility within and between laboratories. Methods: We instigated an international collaborative study of telomere length assessment: 10 different laboratories, employing 3 different techniques [Southern blotting, single telomere length analysis (STELA) and real-time quantitative PCR (qPCR)] performed two rounds of fully blinded measurements on 10 human DNA samples per round to enable unbiased assessment of intra- and inter-batch variation between laboratories and techniques. Results: Absolute results from different laboratories differed widely and could thus not be compared directly, but rankings of relative telomere lengths were highly correlated (correlation coefficients of 0.63-0.99). Intra-technique correlations were similar for Southern blotting and qPCR and were stronger than inter-technique ones. However, inter-laboratory coefficients of variation (CVs) averaged about 10% for Southern blotting and STELA and more than 20% for qPCR. This difference was compensated for by a higher dynamic range for the qPCR method as shown by equal variance after z-scoring. Technical variation per laboratory, measured as median of intra- and inter-batch CVs, ranged from 1.4% to 9.5%, with differences between laboratories only marginally significant (P = 0.06). Gel-based and PCR-based techniques were not different in accuracy. Conclusions: Intra- and inter-laboratory technical variation severely limits the usefulness of data pooling and excludes sharing of reference ranges between laboratories. We propose to establish a common set of physical telomere length standards to improve comparability of telomere length estimates between laboratories.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Hälsovetenskapx Folkhälsovetenskap, global hälsa, socialmedicin och epidemiologi0 (SwePub)303022 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Health Sciencesx Public Health, Global Health, Social Medicine and Epidemiology0 (SwePub)303022 hsv//eng
653 a Ageing
653 a telomeres
653 a variation
653 a biomarker
653 a human
7001 a Baird, Duncan4 aut
7001 a Roger, Laureline4 aut
7001 a Boukamp, Petra4 aut
7001 a Krunic, Damir4 aut
7001 a Cawthon, Richard4 aut
7001 a Dokter, Martin M4 aut
7001 a van der Harst, Pim4 aut
7001 a Bekaert, Sofie4 aut
7001 a de Meyer, Tim4 aut
7001 a Roos, Göranu Umeå universitet,Patologi4 aut0 (Swepub:umu)goro0001
7001 a Svenson, Ulrikau Umeå universitet,Patologi4 aut0 (Swepub:umu)ulason03
7001 a Codd, Veryan4 aut
7001 a Samani, Nilesh J4 aut
7001 a McGlynn, Liane4 aut
7001 a Shiels, Paul G4 aut
7001 a Pooley, Karen A4 aut
7001 a Dunning, Alison M4 aut
7001 a Cooper, Rachel4 aut
7001 a Wong, Andrew4 aut
7001 a Kingston, Andrew4 aut
7001 a von Zglinicki, Thomas4 aut
7102 a Umeå universitetb Patologi4 org
7730 t International Journal of Epidemiologyd : Oxford University Pressg 44:5, s. 1673-1683q 44:5<1673-1683x 0300-5771x 1464-3685
8564 u https://doi.org/10.1093/ije/dyu191y Fulltext
8564 u https://umu.diva-portal.org/smash/get/diva2:897391/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print
8564 u https://doi.org/10.1093/ije/dyu191
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-114645
8564 8u https://doi.org/10.1093/ije/dyu191

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