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Cytokine and antibo...
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Gafvelin, GKarolinska Institutet
(författare)
Cytokine and antibody responses in birch-pollen-allergic patients treated with genetically modified derivatives of the major birch pollen allergen Bet v 1
- Artikel/kapitelEngelska2005
Förlag, utgivningsår, omfång ...
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2005-09-16
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S. Karger AG,2005
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:kth-205258
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https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-205258URI
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https://doi.org/10.1159/000087358DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:1940867URI
Kompletterande språkuppgifter
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Språk:engelska
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Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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QC 20170420
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Background: Recently, recombinant hypoallergenic derivatives of the major birch pollen allergen, Bet v 1, were used to treat birch-pollen-allergic patients in a double-blind, placebo-controlled, multi-centre immunotherapy study. The aim of this study was to evaluate the effects of vaccination with aluminium-hydroxide-adsorbed recombinant Bet v 1 derivatives versus placebo on T-cell, cytokine and antibody responses in a subgroup of patients. Methods: Blood was drawn from patients of the Swedish centre (n=27; rBet v 1 fragments: n=10; rBet v 1 trimer: n=8, and placebo-aluminium hydroxide: n=9) before the start and after completion of the treatment. PBMC were stimulated with rBet v 1 and analysed for cytokine (IL-4, IL-5, IL-10, IL-12, IL-13 and IFN-gamma)-secreting cells by ELISpot. Bet v 1-specific antibody levels in serum (IgG(1-4), IgE and IgA) were measured by ELISA. Skin prick tests with defined Bet v 1 concentrations were performed before and 10-11 months after the beginning of the study. Results: Bet v 1-specific IgG levels, consisting of IgG 1, IgG 2 and IgG 4, were significantly increased after treatment with recombinant allergen derivatives. Treatment with rBet v 1 trimer led to a significant (p<0.05) reduction of Bet v 1-reactive IL-5- and IL-13-producing cells, reflecting a reduced Th2 response. In addition, a decreased number of Bet v 1-reactive IL-4 producing (p=0.07) and an increase of IL-12-producing (p=0.06) cells was noted in the trimer-treated patients. In contrast to placebo, active treatment resulted in significantly reduced immediate-type skin reactions to Bet v 1 even 10-11 months after treatment. Conclusion: Vaccination with recombinant hypoallergenic Bet v 1 derivatives induces a Bet v 1-specific IgG response and leads to reduced skin reactivity in allergic patients. A reduction of Bet v 1-specific Th2 responses was observed in trimer-treated patients, which may reflect the intrinsic property of this allergen derivative. Copyright (C) 2005 S. Karger AG, Basel.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Thunberg, Sarah,1976-Karolinska Institutet(Swepub:kth)u1rw1xrj
(författare)
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Kronqvist, MKarolinska institutet
(författare)
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Gronlund, HKarolinska Institutet
(författare)
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Gronneberg, RKarolinska Institutet
(författare)
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Troye-Blomberg, MKarolinska institutet
(författare)
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Akdis, MSIAF, switzerland
(författare)
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Fiebig, HAllergopharma, Germany
(författare)
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Purohit, AHôpitaux Universitaires de Strasbourg, France
(författare)
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Horak, FMedical University of Vienna, Austria
(författare)
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Reisinger, JMedical University of Vienna, Austria
(författare)
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Niederberger, VMedical University of Vienna, Austria
(författare)
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Akdis, C ASIAF Switzerland
(författare)
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Cromwell, OAllergopharma, Germany
(författare)
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Pauli, GHôpitaux Universitaires de Strasbourg, France
(författare)
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Valenta, RMedical University of Vienna, Austria
(författare)
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van Hage, MKarolinska Institutet
(författare)
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Karolinska InstitutetKarolinska institutet
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:International Archives of Allergy and Immunology: S. Karger AG138:1, s. 59-661018-24381423-0097
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Gafvelin, G
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Thunberg, Sarah, ...
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Kronqvist, M
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Gronlund, H
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Gronneberg, R
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Troye-Blomberg, ...
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visa fler...
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Akdis, M
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Fiebig, H
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Purohit, A
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Horak, F
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Reisinger, J
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Niederberger, V
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Akdis, C A
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Cromwell, O
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Pauli, G
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Valenta, R
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van Hage, M
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