Redistribution of intracellular Ca2+ stores during phagocytosis in human neutrophils

Stendahl, OlleLinköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet (författare)

Subcellular gradients of cytosolic free Ca2+ concentration, [Ca2+]i, are thought to be critical for the localization of functional responses within a cell. A potential but previously unexplored mechanism for the generation of gradients of [Ca2+]i is the accumulation of Ca2+ stores at the site of Ca2+ action. The distribution of the Ca2+ store markers Ca(2+)-dependent adenosine triphosphatase and calreticulin was investigated in resting and phagocytosing human neutrophils. Both proteins showed an evenly distributed fine granular pattern in nonphagocytosing cells, but became markedly concentrated in the filamentous actin-rich cytoplasmic area around the ingested particle during phagocytosis. This redistribution began at early stages of phagocytosis and did not depend on an increase in [Ca2+]i. Thus, accumulation of Ca2+ stores in a restricted area of the cell may contribute to the generation of localized increases in [Ca2+]i.

Krause, Karl-HeinsDivision of Infectious Diseases, Hôpital Cantonal Universitaire, Switzerland (författare)
Krischer, JoachimDepartment of Morphology, Centre Medical Universitaire, Switzerland (författare)
Jerström, PetraLinköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet (författare)
Theler, Jean-MarcClinical Biochemistry, Centre Medical Universitaire, Switzerland (författare)
Clark, Rober A.Department of Medicine, VA Medical Center and University of Iowa, USA (författare)
Carpentier, Jean-LouisDepartment of Morphology, Centre Medical Universitaire, Switzerland (författare)
Lew, Daniel P.Division of Infectious Diseases, Hôpital Cantonal Universitaire, Switzerland (författare)
Linköpings universitetMedicinsk mikrobiologi (creator_code:org_t)

Ingår i:Science: American Association for the Advancement of Science (AAAS)265:5177, s. 1439-14410036-80751095-9203

Av författaren/redakt...: Stendahl, Olle; Krause, Karl-Hei ...; Krischer, Joachi ...; Jerström, Petra; Theler, Jean-Mar ...; Clark, Rober A.; visa fler...; Carpentier, Jean ...; Lew, Daniel P.; visa färre...

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