Tyck till om SwePub Sök
här!
Search: onr:"swepub:oai:DiVA.org:uu-121138" >
Kinetic independenc...
-
Danielson, U HelenaUppsala universitet,Biokemi
(author)
Kinetic independence of the subunits of cytosolic glutathione transferase from the rat
- Article/chapterEnglish1985
Publisher, publication year, extent ...
Numbers
-
LIBRIS-ID:oai:DiVA.org:uu-121138
-
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-121138URI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
The steady-state kinetics of the dimeric glutathione transferases deviate from Michaelis-Menten kinetics, but have hyperbolic binding isotherms for substrates and products of the enzymic reaction. The possibility of subunit interactions during catalysis as an explanation for the rate behaviour was investigated by use of rat isoenzymes composed of subunits 1, 2, 3 and 4, which have distinct substrate specificities. The kinetic parameter kcat./Km was determined with 1-chloro-2,4-dinitrobenzene, 4-hydroxyalk-2-enals, ethacrynic acid and trans-4-phenylbut-3-en-2-one as electrophilic substrates for six isoenzymes: rat glutathione transferases 1-1, 1-2, 2-2, 3-3, 3-4 and 4-4. It was found that the kcat./Km values for the heterodimeric transferases 1-2 and 3-4 could be predicted from the kcat./Km values of the corresponding homodimers. Likewise, the initial velocities determined with transferases 3-3, 3-4 and 4-4 at different degrees of saturation with glutathione and 1-chloro-2,4-dinitrobenzene demonstrated that the kinetic properties of the subunits are additive. These results show that the subunits of glutathione transferase are kinetically independent.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Mannervik, Bengt
(author)
-
Uppsala universitetBiokemi
(creator_code:org_t)
Related titles
-
In:Biochemical Journal231:2, s. 263-2670264-60211470-8728
Internet link
Find in a library
To the university's database