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  • Jensen, Pernille LinnertLaboratory of Reproductive Biology, University Hospital of Copenhagen, Denmark (author)

Proteomic Analysis of Human Blastocoel Fluid and Blastocyst Cells

  • Article/chapterEnglish2013

Publisher, publication year, extent ...

  • Mary Ann Liebert Inc,2013
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:uu-187067
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-187067URI
  • https://doi.org/10.1089/scd.2012.0239DOI

Supplementary language notes

  • Language:English
  • Summary in:English

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Human embryonic stem cells (hESCs) are derived from the inner cell mass (ICM) of the blastocyst and can differentiate into any cell type in the human body. These cells hold a great potential for regenerative medicine, but in order to obtain enough cells needed for medical treatment, culture is required on a large scale. In the undifferentiated state, hESCs appear to possess an unlimited potential for proliferation but optimal, defined and safe culture conditions remains a challenge. The aim of the present study was to identify proteins in the natural environment of undifferentiated hESCs, namely the blastocoel fluid, which is in contact with all the cells in the blastocyst, including hESCs. Fifty-three surplus human blastocysts were donated after informed consent and blastocoel fluid was isolated by micromanipulation. Using highly sensitive nano high pressure liquid chromatography tandem mass spectrometry, 286 proteins were identified in the blastocoel fluid and 1307 proteins in the corresponding cells of the blastocyst. Forty-two were previously uncharacterized proteins - eight of these originated from the blastocoel fluid. Furthermore, several heat shock proteins (Hsp27, Hsp60, Hsc70 and Hsp90) were identified in blastocoel fluid together with zona pellucida proteins (ZP2-4), Vitamin D binding protein and Retinol binding protein. Proteins that regulate ciliary assembly and function were also identified, including Bardet-biedl syndrome protein 7. This study has identified numerous proteins which cells from the ICM of the human blastocyst are exposed to via the blastocoel fluid. These results can be an inspiration for the development of improved culture conditions for hESCs.

Added entries (persons, corporate bodies, meetings, titles ...)

  • Beck, Hans Christianentre for Clinical Proteomics, Department of Clinical Biochemistry and Pharmacology, Odense University Hospital, Denmark (author)
  • Petersen, JørgenDepartment of Biochemistry and Molecular Biology, University of Southern Denmark (author)
  • Hreinsson, Julius,1963-Uppsala universitet,Obstetrik & gynekologi,Klinisk och experimentell reproduktionsbiologi/Olovsson(Swepub:uu)julhr616 (author)
  • Wånggren, Kjell,1954-Uppsala universitet,Obstetrik & gynekologi,Klinisk och experimentell reproduktionsbiologi/Olovsson(Swepub:uu)kjewa145 (author)
  • Laursen, Steen BFertility clinic IVF-SYD, Denmark (author)
  • Sørensen, Pernille DissingORIGIO a/s, Denmark (author)
  • Christensen, Søren TDepartment of Biology, Section of Cell and Developmental Biology, University of Copenhagen, Denmark (author)
  • Andersen, Claus YdingLaboratory of Reproductive Biology, University Hospital of Copenhagen, Denmark (author)
  • Laboratory of Reproductive Biology, University Hospital of Copenhagen, Denmarkentre for Clinical Proteomics, Department of Clinical Biochemistry and Pharmacology, Odense University Hospital, Denmark (creator_code:org_t)

Related titles

  • In:Stem Cells and Development: Mary Ann Liebert Inc22:7, s. 1126-11351547-32871557-8534

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