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Multi-HLA class II ...
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Gerstner, ChristinaKarolinska Univ Hosp, Ctr Mol Med, Dept Med Solna, Div Rheumatol, Stockholm, Sweden.;Karolinska Inst, Stockholm, Sweden.
(författare)
Multi-HLA class II tetramer analyses of citrulline-reactive T cells and early treatment response in rheumatoid arthritis
- Artikel/kapitelEngelska2020
Förlag, utgivningsår, omfång ...
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2020-05-18
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Springer Science and Business Media LLC,2020
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electronicrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:uu-414324
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-414324URI
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https://doi.org/10.1186/s12865-020-00357-wDOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:143813072URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Background HLA class II tetramers can be used for ex vivo enumeration and phenotypic characterisation of antigen-specific CD4+ T cells. They are increasingly applied in settings like allergy, vaccination and autoimmune diseases. Rheumatoid arthritis (RA) is a chronic autoimmune disorder for which many autoantigens have been described. Results Using multi-parameter flow cytometry, we developed a multi-HLA class II tetramer approach to simultaneously study several antigen specificities in RA patient samples. We focused on previously described citrullinated HLA-DRB1*04:01-restricted T cell epitopes from alpha-enolase, fibrinogen-beta, vimentin as well as cartilage intermediate layer protein (CILP). First, we examined inter-assay variability and the sensitivity of the assay in peripheral blood from healthy donors (n = 7). Next, we confirmed the robustness and sensitivity in a cohort of RA patients with repeat blood draws (n = 14). We then applied our method in two different settings. We assessed lymphoid tissue from seropositive arthralgia (n = 5) and early RA patients (n = 5) and could demonstrate autoreactive T cells in individuals at risk of developing RA. Lastly, we studied peripheral blood from early RA patients (n = 10) and found that the group of patients achieving minimum disease activity (DAS28 < 2.6) at 6 months follow-up displayed a decrease in the frequency of citrulline-specific T cells. Conclusions Our study demonstrates the development of a sensitive tetramer panel allowing simultaneous characterisation of antigen-specific T cells in ex vivo patient samples including RA 'at risk' subjects. This multi-tetramer approach can be useful for longitudinal immune-monitoring in any disease with known HLA-restriction element and several candidate antigens.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Turcinov, SaraKarolinska Institutet
(författare)
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Hensvold, Aase H.Karolinska Institutet
(författare)
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Chemin, KarineKarolinska Institutet
(författare)
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Uchtenhagen, HannesKarolinska Univ Hosp, Ctr Mol Med, Dept Med Solna, Div Rheumatol, Stockholm, Sweden.;Karolinska Inst, Stockholm, Sweden.;BRI Virginia Mason, Translat Res Program, Seattle, WA USA.
(författare)
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Ramwadhdoebe, Tamara H.Univ Amsterdam, Amsterdam UMC, Dept Clin Immunol & Rheumatol, Amsterdam Infect & Immun Inst, Amsterdam, Netherlands.;Univ Amsterdam, Amsterdam UMC, Dept Expt Immunol, Amsterdam Infect & Immun Inst, Amsterdam, Netherlands.;Acad Med Ctr, Amsterdam Rheumatol & Immunol Ctr ARC, Amsterdam, Netherlands.
(författare)
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Dubnovitsky, AnatolyKarolinska Institutet
(författare)
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Kozhukh, GenadiyKarolinska Univ Hosp, Ctr Mol Med, Dept Med Solna, Div Rheumatol, Stockholm, Sweden.;Karolinska Inst, Stockholm, Sweden.
(författare)
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Rönnblom, LarsUppsala universitet,Reumatologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)larsronn
(författare)
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Kwok, William W.BRI Virginia Mason, Translat Res Program, Seattle, WA USA.
(författare)
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Achour, AdnaneKarolinska Institutet
(författare)
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Catrina, AncaKarolinska Univ Hosp, Ctr Mol Med, Dept Med Solna, Div Rheumatol, Stockholm, Sweden.;Karolinska Inst, Stockholm, Sweden.
(författare)
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van Baarsen, Lisa G. M.Karolinska Institutet
(författare)
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Malmstrom, VivianneKarolinska Univ Hosp, Ctr Mol Med, Dept Med Solna, Div Rheumatol, Stockholm, Sweden.;Karolinska Inst, Stockholm, Sweden.
(författare)
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Karolinska InstitutetKarolinska Univ Hosp, Ctr Mol Med, Dept Med Solna, Div Rheumatol, Stockholm, Sweden.;Karolinska Inst, Stockholm, Sweden.
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:BMC Immunology: Springer Science and Business Media LLC21:11471-2172
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Gerstner, Christ ...
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Turcinov, Sara
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Hensvold, Aase H ...
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Chemin, Karine
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Uchtenhagen, Han ...
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Ramwadhdoebe, Ta ...
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visa fler...
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Dubnovitsky, Ana ...
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Kozhukh, Genadiy
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Rönnblom, Lars
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Kwok, William W.
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Achour, Adnane
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Catrina, Anca
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van Baarsen, Lis ...
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Malmstrom, Vivia ...
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