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Human eosinophil pe...
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Carlson, MUppsala universitet,Institutionen för medicinska vetenskaper
(författare)
Human eosinophil peroxidase : purification and characterization
- Artikel/kapitelEngelska1985
Förlag, utgivningsår, omfång ...
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:uu-74328
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-74328URI
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Språk:engelska
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Sammanfattning på:engelska
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Klassifikation
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Human eosinophil peroxidase (EPO) was isolated from granules from granulocytes of a patient with hypereosinophilia. The granules were extracted by means of 0.2 M NaAc, pH 4.0. The purification steps included gel filtration chromatography on Sephadex G-75 superfine and ion-exchange chromatography on CM-Sephadex G-50. The purified protein showed one band on agarose-electrophoresis, a high peroxidase activity, and a 415-nm/280 nm ratio of 1.15. After reduction, EPO showed two bands on SDS-PAGE of m.w. 52,000 and 15,000, respectively. On gel filtration, the unreduced protein had a m.w. of approximately 77,000. Amino acid analyses showed a high content of arginine and aspartic acid. Monospecific antibodies to EPO were prepared in rabbits, and a specific radioimmunoassay was developed. There was an almost linear correlation between the content of EPO measured by the radioimmunoassay and the number of eosinophils in a mixed cell extract from reference material, indicating the eosinophil origin of EPO. The content of EPO was estimated to be 15.0 micrograms/10(6) eosinophils.
Ämnesord och genrebeteckningar
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Amino Acids/analysis
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Antigen-Antibody Reactions
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Chromatography; Gel
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Eosinophil Peroxidase
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Eosinophilia/enzymology
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Eosinophils/*enzymology
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Humans
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Immunodiffusion
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Leukocyte Count
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Molecular Weight
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Peroxidases/analysis/*isolation & purification/metabolism
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Radioimmunoassay
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Research Support; Non-U.S. Gov't
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MEDICINE
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MEDICIN
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Peterson, C GUppsala universitet,Institutionen för medicinska vetenskaper
(författare)
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Venge, PUppsala universitet,Institutionen för medicinska vetenskaper(Swepub:uu)pervenge
(författare)
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Uppsala universitetInstitutionen för medicinska vetenskaper
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Journal of Immunology134:3, s. 1875-18790022-17671550-6606
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