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- Yu, Miao meiThird Affiliated Hospital of Soochow University (author)
Apolipoprotein M increases the expression of Vitamin D receptor mRNA in colorectal cancer cells detected with duplex fluorescence reverse transcription-quantitative polymerase chain reaction
- Article/chapterEnglish2017
Publisher, publication year, extent ...
- 2017-06-07
- Spandidos Publications,2017
- 6 s.
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- LIBRIS-ID:oai:lup.lub.lu.se:99d4562a-8724-4c84-a80a-dd3c6deec1ff
- https://lup.lub.lu.se/record/99d4562a-8724-4c84-a80a-dd3c6deec1ffURI
- https://doi.org/10.3892/mmr.2017.6716DOI
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- Language:English
- Summary in:English
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- Apolipoprotein M (ApoM) and the Vitamin D receptor (VDR) are apolipoproteins predominantly presenting in high-density lipoprotein (HDL) and a karyophilic protein belonging to the steroid-thyroid receptor superfamily, respectively. Previous studies have demonstrated that ApoM and VDR are associated with cholesterol metabolism, immune and colorectal cancer regulation. In order to investigate whether ApoM affected the expression of VDR in colorectal cancer cells, a single-tube duplex fluorescence reverse transcription-quantitative polymerase chain reaction (RT-qPCR) system was developed to simultaneously detect the mRNA levels of VDR and GAPDH in HT-29 cells overexpressing ApoM. The results demonstrated that the amplification products were confirmed as the specific fragment of VDR/GAPDH using the DNA sequencing instrument. The sensitivity, linear range, correlation coefficient, amplification efficiency, intra-assay and inter-assay coefficients of variation were 40 copies/μl, 4.00×101-4.00×105 copies/μl, 0.999, 92.42%, 0.09-0.34% and 0.32-0.65% for VDR, and 40 copies/μl, 400×101-4.00×105 copies/μl, 0.999, 98.07%, 0.19-0.43% and 0.40-0.75% for GAPDH, respectively. The results indicated that the expression of VDR mRNA was significantly higher in HT-29 cells overexpressing ApoM, compared with the negative control group (P<0.05). In conclusion, the current study successfully developed the single-tube duplex RT-qPCR to simultaneously detect VDR and GAPDH expression in colorectal cancer cells. The methodology results demonstrated that the duplex RT-qPCR system with high sensitivity and specificity could ensure the objectivity and credibility of the detection. The present study confirmed that ApoM significantly increased the expression of VDR in HT-29 cells. In addition, it was hypothesized that ApoM may be involved in antineoplastic activity via the upregulation of VDR expression, which may provide novel directions for the investigation of ApoM in cancer.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Cancer och onkologi hsv//swe
- MEDICAL AND HEALTH SCIENCES Clinical Medicine Cancer and Oncology hsv//eng
- Apolipoprotein M
- Colorectal cancer
- Glyceraldehyde-3-phosphate dehydrogenase
- Polymerase chain reaction
- QPCR
- Vitamin D receptor
Added entries (persons, corporate bodies, meetings, titles ...)
- Yao, ShuangThird Affiliated Hospital of Soochow University (author)
- Luo, Kai MingThird Affiliated Hospital of Soochow University (author)
- Mu, Qin fengThird Affiliated Hospital of Soochow University (author)
- Yu, YangThird Affiliated Hospital of Soochow University (author)
- Luo, Guang-HuaThird Affiliated Hospital of Soochow University (author)
- Xu, NingLund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)kkem-nxu (author)
- Third Affiliated Hospital of Soochow UniversityAvdelningen för klinisk kemi och farmakologi (creator_code:org_t)
Related titles
- In:Molecular Medicine Reports: Spandidos Publications16:2, s. 1167-11721791-29971791-3004
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