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Formation of Kv2.1-...
Formation of Kv2.1-FAK complex as a mechanism of FAK activation, cell polarization and enhanced motility
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Wei, Jian-Feng (författare)
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Wei, Ling (författare)
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Zhou, Xin (författare)
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Lu, Zhong-Yang (författare)
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Francis, Kevin (författare)
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Hu, Xin-Yang (författare)
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Liu, Yu (författare)
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Xiong, Wen-Cheng (författare)
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- Zhang, Xiao (författare)
- Uppsala universitet,Institutionen för folkhälso- och vårdvetenskap
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Banik, Naren L. (författare)
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Zheng, Shu-Sen (författare)
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Yu, Shan Ping (författare)
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(creator_code:org_t)
- Wiley, 2008
- 2008
- Engelska.
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Ingår i: Journal of Cellular Physiology. - : Wiley. - 0021-9541 .- 1097-4652. ; 217:2, s. 544-557
- Relaterad länk:
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https://europepmc.or...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Focal adhesion kinase (FAK) plays key roles in cell adhesion and migration. We now report that the delayed rectifier Kv2.1 potassium channel, through its LD-like motif in N-terminus, may interact with FAK and enhance phosphorylation of FAK(397) and FAK(576/577) Overlapping distribution of Kv2.1 and FAK was observed on soma and proximal dendrites of cortical neurons. FAK expression promotes a polarized membrane distribution of the Kv2.1 channel. In Kv2.1-transfected CHO cells, formation of the Kv2.1-FAK complex was stimulated by fibronectin/integrin and inhibited by the K channel blocker tetraethylammonium (TEA). FAK phosphorylation was minimized by shRNA knockdown of the Kv2.1 channel, point mutations of the N-terminus, and TEA, respectively. Cell migration morphology was altered by Kv2.1 knockdown or TEA, hindering cell migration activity. In wound healing tests in vitro and a traumatic injury animal model, Kv2.1 expression and co-localization of Kv2.1 and FAK significantly enhanced directional cell migration and wound closure. It is suggested that the Kv2.1 channel may function as a promoting signal for FAK activation and cell motility.
Nyckelord
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Wei, Jian-Feng
-
Wei, Ling
-
Zhou, Xin
-
Lu, Zhong-Yang
-
Francis, Kevin
-
Hu, Xin-Yang
-
visa fler...
-
Liu, Yu
-
Xiong, Wen-Cheng
-
Zhang, Xiao
-
Banik, Naren L.
-
Zheng, Shu-Sen
-
Yu, Shan Ping
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visa färre...
- Artiklar i publikationen
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Journal of Cellu ...
- Av lärosätet
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Uppsala universitet