Sökning: onr:"swepub:oai:DiVA.org:uu-157259" >
Structural Alterati...
Structural Alterations from Multiple Displacement Amplification of a Human Genome Revealed by Mate-Pair Sequencing
-
- Jiao, Xiang (författare)
- Uppsala universitet,Genomik
-
- Rosenlund, Magnus (författare)
- Uppsala universitet,Genomik
-
- Hooper, Sean D. (författare)
- Uppsala universitet,Genomik
-
visa fler...
-
- Tellgren-Roth, Christian (författare)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi
-
He, Liqun (författare)
-
Fu, Yutao (författare)
-
Mangion, Jonathan (författare)
-
- Sjöblom, Tobias (författare)
- Uppsala universitet,Genomik
-
visa färre...
-
(creator_code:org_t)
- 2011-07-22
- 2011
- Engelska.
-
Ingår i: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 6:7, s. e22250-
- Relaterad länk:
-
https://uu.diva-port... (primary) (Raw object)
-
visa fler...
-
https://journals.plo...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Comprehensive identification of the acquired mutations that cause common cancers will require genomic analyses of large sets of tumor samples. Typically, the tissue material available from tumor specimens is limited, which creates a demand for accurate template amplification. We therefore evaluated whether phi29-mediated whole genome amplification introduces false positive structural mutations by massive mate-pair sequencing of a normal human genome before and after such amplification. Multiple displacement amplification led to a decrease in clone coverage and an increase by two orders of magnitude in the prevalence of inversions, but did not increase the prevalence of translocations. While multiple strand displacement amplification may find uses in translocation analyses, it is likely that alternative amplification strategies need to be developed to meet the demands of cancer genomics.
Nyckelord
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
-
PLOS ONE
(Sök värdpublikationen i LIBRIS)
Till lärosätets databas