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Evaluation of the tissue microarray technique for immunohistochemical analysis in rectal cancer.
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- Fernebro, Eva (författare)
- Lund University,Lunds universitet,Bröstcancer-genetik,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Breastcancer-genetics,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Dictor, Michael (författare)
- Lund University,Lunds universitet,Tumörmikromiljö,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Tumor microenvironment,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine
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- Bendahl, Pär-Ola (författare)
- Lund University,Lunds universitet,Bröstcancer-genetik,Sektion I,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Breastcancer-genetics,Section I,Department of Clinical Sciences, Lund,Faculty of Medicine
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- (creator_code:org_t)
- 2002
- 2002
- Engelska.
- Ingår i: Archives of pathology & laboratory medicine. - 0003-9985. ; 126:6, s. 702-705
- Tidskriftsartikel (refereegranskat)
Abstract
Ämnesord
Stäng
- BACKGROUND: Immunohistochemical staining for tumor-associated proteins is widely used for the identification of novel prognostic markers. Recently, a tissue-conserving, high-throughput technique, tissue microarray, has been introduced. This technique uses 0.6-mm tissue core biopsy specimens, 500 to 1000 of which are brought into a new paraffin array block, which can be sectioned up to 100 times. METHODS: We evaluated the tissue microarray technique for immunohistochemical analysis in 20 rectal cancers. Immunohistochemical staining was performed for the proliferation marker Ki-67 and the tumor suppressor protein p53 in whole tissue sections and in tissue core biopsy specimens. RESULTS: The whole tissue sections were assessed by counting all cells in 10 high-power fields (x40), which resulted in a mean fraction of Ki-67-expressing tumor cells of 0.81 (range, 0.54-1.0). p53 expression assessed in whole tissue sections showed nuclear staining in 15 (75%) of 20 rectal carcinomas. For the tissue microarray technique, a median of 3 (range, 3-5) 0.6-mm tissue core biopsy specimens were studied from each of the 20 tumor specimens. The tissue microarray method gave a mean Ki-67 expression of 0.85 (range, 0.50-1.0) in tumor cell nuclei and showed p53 protein expression in the same 15 of 20 tumors as in the whole tissue sections. CONCLUSION: We conclude that the tissue microarray technique for immunohistochemical staining in rectal cancer yields staining of good quality and expression data for Ki-67 and p53 comparable to those obtained with whole tissue staining. The feasibility of tissue microarray thus enables time- and tissue-preserving studies of multiple markers in large tumor series.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Nyckelord
- Rectal Neoplasms : chemistry
- Protein p53 : analysis
- Middle Age
- Male
- Ki-67 Antigen : analysis
- Immunoenzyme Techniques
- Human
- Histocytological Preparation Techniques : methods
- Female
- Cell Count
- Carcinoma : pathology
- Carcinoma : chemistry
- Needle
- Biopsy
- Adult
- Aged
- Rectal Neoplasms : pathology
- Support
- Non-U.S. Gov't
- Tumor Markers
- Biological : analysis
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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- Archives of pathology & laboratory medicine (Sök värdpublikationen i LIBRIS)
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- Fernebro, Eva
- Dictor, Michael
- Bendahl, Pär-Ola
- Fernö, Mårten
- Nilbert, Mef
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- MEDICIN OCH HÄLSOVETENSKAP
- MEDICIN OCH HÄLS ...
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- Lunds universitet
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