Sökning: onr:"swepub:oai:DiVA.org:su-96106" > Multiplex detection...
Fältnamn | Indikatorer | Metadata |
---|---|---|
000 | 03351naa a2200457 4500 | |
001 | oai:DiVA.org:su-96106 | |
003 | SwePub | |
008 | 131111s2013 | |||||||||||000 ||eng| | |
009 | oai:DiVA.org:uu-210582 | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-961062 URI |
024 | 7 | a https://doi.org/10.1016/j.diagmicrobio.2013.06.0132 DOI |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-2105822 URI |
040 | a (SwePub)sud (SwePub)uu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Barisic, Ivan4 aut |
245 | 1 0 | a Multiplex detection of antibiotic resistance genes using padlock probes |
264 | 1 | b Elsevier BV,c 2013 |
338 | a print2 rdacarrier | |
500 | a AuthorCount:6; | |
520 | a The elucidation of resistance mechanisms is of central importance to providing and maintaining efficient medical treatment. However, molecular detection methods covering the complete set of resistance genes with a single test are still missing. Here, we present a novel 100-plex assay based on padlock probes in combination with a microarray that allows the simultaneous large-scale identification of highly diverse beta-lactamases. The specificity of the assay was performed using 70 clinical bacterial isolates, recovering 98% of the beta-lactamase nucleotide sequences present. Additionally, the sensitivity was evaluated with PCR products and genomic bacterial DNA, revealing a detection limit of 10(4) DNA copies per reaction when using PCR products as the template. Pre-amplification of genomic DNA in a 25-multiplex PCR further facilitated the detection of beta-lactamase genes in dilutions of 10(7) cells/mL. In summary, we present an efficient, highly specific, and highly sensitive multiplex detection method for any gene. | |
650 | 7 | a NATURVETENSKAPx Biologix Mikrobiologi0 (SwePub)106062 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Microbiology0 (SwePub)106062 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Infektionsmedicin0 (SwePub)302092 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Infectious Medicine0 (SwePub)302092 hsv//eng |
653 | a Multiplex detection | |
653 | a beta-lactamases | |
653 | a Microarray | |
653 | a Padlock probes | |
700 | 1 | a Schoenthaler, Silvia4 aut |
700 | 1 | a Ke, Rongqinu Uppsala universitet,Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),Institutionen för immunologi, genetik och patologi,Rudbeck Laboratory4 aut |
700 | 1 | a Nilsson, Matsu Uppsala universitet,Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),Molekylära verktyg,Rudbeck Laboratory4 aut0 (Swepub:uu)matsnils |
700 | 1 | a Noehammer, Christa4 aut |
700 | 1 | a Wiesinger-Mayr, Herbert4 aut |
710 | 2 | a Stockholms universitetb Institutionen för biokemi och biofysik4 org |
773 | 0 | t Diagnostic microbiology and infectious diseased : Elsevier BVg 77:2, s. 118-125q 77:2<118-125x 0732-8893x 1879-0070 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-96106 |
856 | 4 8 | u https://doi.org/10.1016/j.diagmicrobio.2013.06.013 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-210582 |
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
Kopiera och spara länken för att återkomma till aktuell vy