Sökning: onr:"swepub:oai:DiVA.org:umu-114645" > Reproducibility of ...
Fältnamn | Indikatorer | Metadata |
---|---|---|
000 | 04432naa a2200637 4500 | |
001 | oai:DiVA.org:umu-114645 | |
003 | SwePub | |
008 | 160125s2015 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1146452 URI |
024 | 7 | a https://doi.org/10.1093/ije/dyu1912 DOI |
040 | a (SwePub)umu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Martin-Ruiz, Carmen M4 aut |
245 | 1 0 | a Reproducibility of telomere length assessment :b an international collaborative study |
264 | c 2014-09-19 | |
264 | 1 | b Oxford University Press,c 2015 |
338 | a electronic2 rdacarrier | |
520 | a Background: Telomere length is a putative biomarker of ageing, morbidity and mortality. Its application is hampered by lack of widely applicable reference ranges and uncertainty regarding the present limits of measurement reproducibility within and between laboratories. Methods: We instigated an international collaborative study of telomere length assessment: 10 different laboratories, employing 3 different techniques [Southern blotting, single telomere length analysis (STELA) and real-time quantitative PCR (qPCR)] performed two rounds of fully blinded measurements on 10 human DNA samples per round to enable unbiased assessment of intra- and inter-batch variation between laboratories and techniques. Results: Absolute results from different laboratories differed widely and could thus not be compared directly, but rankings of relative telomere lengths were highly correlated (correlation coefficients of 0.63-0.99). Intra-technique correlations were similar for Southern blotting and qPCR and were stronger than inter-technique ones. However, inter-laboratory coefficients of variation (CVs) averaged about 10% for Southern blotting and STELA and more than 20% for qPCR. This difference was compensated for by a higher dynamic range for the qPCR method as shown by equal variance after z-scoring. Technical variation per laboratory, measured as median of intra- and inter-batch CVs, ranged from 1.4% to 9.5%, with differences between laboratories only marginally significant (P = 0.06). Gel-based and PCR-based techniques were not different in accuracy. Conclusions: Intra- and inter-laboratory technical variation severely limits the usefulness of data pooling and excludes sharing of reference ranges between laboratories. We propose to establish a common set of physical telomere length standards to improve comparability of telomere length estimates between laboratories. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Hälsovetenskapx Folkhälsovetenskap, global hälsa, socialmedicin och epidemiologi0 (SwePub)303022 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Health Sciencesx Public Health, Global Health, Social Medicine and Epidemiology0 (SwePub)303022 hsv//eng |
653 | a Ageing | |
653 | a telomeres | |
653 | a variation | |
653 | a biomarker | |
653 | a human | |
700 | 1 | a Baird, Duncan4 aut |
700 | 1 | a Roger, Laureline4 aut |
700 | 1 | a Boukamp, Petra4 aut |
700 | 1 | a Krunic, Damir4 aut |
700 | 1 | a Cawthon, Richard4 aut |
700 | 1 | a Dokter, Martin M4 aut |
700 | 1 | a van der Harst, Pim4 aut |
700 | 1 | a Bekaert, Sofie4 aut |
700 | 1 | a de Meyer, Tim4 aut |
700 | 1 | a Roos, Göranu Umeå universitet,Patologi4 aut0 (Swepub:umu)goro0001 |
700 | 1 | a Svenson, Ulrikau Umeå universitet,Patologi4 aut0 (Swepub:umu)ulason03 |
700 | 1 | a Codd, Veryan4 aut |
700 | 1 | a Samani, Nilesh J4 aut |
700 | 1 | a McGlynn, Liane4 aut |
700 | 1 | a Shiels, Paul G4 aut |
700 | 1 | a Pooley, Karen A4 aut |
700 | 1 | a Dunning, Alison M4 aut |
700 | 1 | a Cooper, Rachel4 aut |
700 | 1 | a Wong, Andrew4 aut |
700 | 1 | a Kingston, Andrew4 aut |
700 | 1 | a von Zglinicki, Thomas4 aut |
710 | 2 | a Umeå universitetb Patologi4 org |
773 | 0 | t International Journal of Epidemiologyd : Oxford University Pressg 44:5, s. 1673-1683q 44:5<1673-1683x 0300-5771x 1464-3685 |
856 | 4 | u https://doi.org/10.1093/ije/dyu191y Fulltext |
856 | 4 | u https://umu.diva-portal.org/smash/get/diva2:897391/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print |
856 | 4 | u https://doi.org/10.1093/ije/dyu191 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-114645 |
856 | 4 8 | u https://doi.org/10.1093/ije/dyu191 |
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
Kopiera och spara länken för att återkomma till aktuell vy